Department of Chemistry, University of Waterloo, Ontario, Canada N2L 3G1. janusz@uwaterloo.
Analyst. 2017 Aug 7;142(16):2928-2935. doi: 10.1039/c7an00718c.
On-site screening for target analytes in complex matrices, such as biofluids and food specimens, not only requires reliable and portable analytical instrumentation, but also solvent-free and easy-to-use sampling/sample preparation approaches that allow analytes of interest to be isolated from such matrices. The integration of sampling devices with field deployable instruments should be as efficient as possible, and should aim to provide rapid, precise, and accurate results that enable quick on-site decision. In this study, we evaluated solid-phase microextraction-transmission (SPME-TM) coupled to a portable single quadrupole MS system, via direct analysis in real time (DART), as an effective tool for the rapid screening of target analytes in biological and food matrices. Limits of quantitation (LOQ) in the low parts-per-billion levels (≤50 ng mL) were attained for most of the investigated analytes with total analysis times under 2 min per sample. Furthermore, we explored the suitability of this technology for on-site rapid molecular profiling of complex matrices. As a proof-of-concept, we demonstrate the rapid identification of milk samples from assorted animal and vegetal sources.
现场分析复杂基质（如生物体液和食品样本）中的目标分析物，不仅需要可靠且便携的分析仪器，还需要无溶剂且易于使用的采样/样品制备方法，以便将感兴趣的分析物从这些基质中分离出来。采样设备与现场部署仪器的集成应尽可能高效，并旨在提供快速、精确和准确的结果，以便能够快速做出现场决策。在这项研究中，我们通过实时直接分析（DART）评估了固相微萃取传输（SPME-TM）与便携式单四极杆 MS 系统的联用，作为一种快速筛选生物和食品基质中目标分析物的有效工具。大多数研究分析物的定量下限（LOQ）在低纳克/毫升水平（≤50 ng/mL），每个样品的总分析时间不到 2 分钟。此外，我们还探索了该技术用于现场快速分析复杂基质的分子特征的适用性。作为概念验证，我们展示了快速识别来自各种动物和植物来源的牛奶样本的能力。
