Department of Chemistry, University of Waterloo, 200 University Avenue, Waterloo, N2L 3G1, Canada.
Angew Chem Int Ed Engl. 2016 Jun 20;55(26):7510-4. doi: 10.1002/anie.201601476. Epub 2016 May 9.
Herein we report the development of solid-phase microextraction (SPME) devices designed to perform fast extraction/enrichment of target analytes present in small volumes of complex matrices (i.e. V≤10 μL). Micro-sampling was performed with the use of etched metal tips coated with a thin layer of biocompatible nano-structured polypyrrole (PPy), or by using coated blade spray (CBS) devices. These devices can be coupled either to liquid chromatography (LC), or directly to mass spectrometry (MS) via dedicated interfaces. The reported results demonstrated that the whole analytical procedure can be carried out within a few minutes with high sensitivity and quantitation precision, and can be used to sample from various biological matrices such as blood, urine, or Allium cepa L single-cells.
本文报告了固相微萃取（SPME）装置的开发，该装置旨在对小体积复杂基质（即 V≤10 μL）中存在的目标分析物进行快速萃取/富集。微采样是用涂有一层薄的生物相容性纳米结构聚吡咯（PPy）的刻蚀金属尖端或使用涂覆刀片喷雾（CBS）装置进行的。这些装置可以通过专用接口与液相色谱（LC）或直接与质谱（MS）联用。所报道的结果表明，整个分析过程可以在几分钟内完成，具有高灵敏度和定量精度，并且可以用于从各种生物基质（如血液、尿液或洋葱单个细胞）中采样。
