Gustina Sri, Hasbi Hasbi, Karja Ni Wayan Kurniani, Setiadi Mohamad Agus, Supriatna Iman
Division of Reproduction and Obstetrics, Department of Veterinary Clinic, Reproduction and Pathology, Bogor Agricultural University, Bogor, Jawa Barat, Indonesia.
Division of Animal Science, Faculty of Animal Science and Fisheries, Sulawesi Barat University, Majene, Sulawesi Barat, Indonesia.
Anim Sci J. 2017 Dec;88(12):1911-1915. doi: 10.1111/asj.12839. Epub 2017 Jul 19.
The aim of this research was to identify the changes in the cytoplasmic ultrastructure of immature and matured oocytes in buffalo (Bubalus bubalis). Oocytes were matured in vitro in tissue culture medium-199 with and without sericin, and then analyzed by light and transmission electron microscopy. The experiment result showed that the nuclear maturation rate of buffalo oocytes was significantly higher in the presence of sericin (80.6%) than without sericin (68.1%) (P < 0.05). The immature oocytes were characterized by cortical granule clusters in the ooplasm and the absence of perivitelline space (PVS). In contrast, the oocytes matured either with or without sericin showed the formation of PVS, erected microvilli, the migration of cortical granules to the cytoplasmic periphery, and the clear appearance of the mitochondria and vesicle in the oolemma. Interestingly, matured oocytes with sericin have smaller cortical granules than do immature oocytes (P < 0.05). In conclusion, supplementation of 0.05% sericin in the maturation medium can enhance the maturation rate of buffalo oocytes. Several cytoplasmic ultrastructures were relocated and modulated during the in vitro maturation process of buffalo oocytes: PVS development, cortical granules migration to periphery, and mitochondria and vesicles in the cortical region. The ultrastructure was similar between the groups with and without sericin.
本研究的目的是确定水牛（Bubalus bubalis）未成熟和成熟卵母细胞细胞质超微结构的变化。卵母细胞在含有和不含丝胶蛋白的199组织培养基中进行体外成熟，然后通过光学显微镜和透射电子显微镜进行分析。实验结果表明，存在丝胶蛋白时水牛卵母细胞的核成熟率（80.6%）显著高于不存在丝胶蛋白时（68.1%）（P < 0.05）。未成熟卵母细胞的特征是卵质中有皮质颗粒簇且不存在卵周隙（PVS）。相反，无论有无丝胶蛋白成熟的卵母细胞均显示出PVS的形成、微绒毛竖起、皮质颗粒向细胞质周边迁移以及卵膜中线粒体和囊泡的清晰出现。有趣的是，有丝胶蛋白的成熟卵母细胞的皮质颗粒比未成熟卵母细胞的小（P < 0.05）。总之，在成熟培养基中添加0.05%的丝胶蛋白可提高水牛卵母细胞的成熟率。在水牛卵母细胞的体外成熟过程中，几种细胞质超微结构发生了重新定位和调节：PVS发育、皮质颗粒向周边迁移以及皮质区域中的线粒体和囊泡。有丝胶蛋白组和无丝胶蛋白组之间的超微结构相似。
