西澳大利亚犬类中[物种名称未给出]的血清学证据。
Serological Evidence of spp. in Western Australian Dogs.
作者信息
Bennett Mark David, Abdad Mohammad Yazid, Stenos John
机构信息
Murdoch University, Western Australia, Australia.
Australian Rickettsial Reference Laboratory, University Hospital Geelong, Victoria, Australia.
出版信息
Am J Trop Med Hyg. 2017 Aug;97(2):407-412. doi: 10.4269/ajtmh.16-0998. Epub 2017 Jul 19.
It has been claimed that dogs can be useful sentinels for public health monitoring of vector-borne infectious diseases, including spp. We used 153 canine blood samples opportunistically collected at Murdoch University Veterinary Hospital and 156 canine sera collected from Aboriginal communities in northwest Western Australia to test for evidence of spp. exposure, using microimmunofluorescence (MIF) in the latter case, and both MIF and polymerase chain reaction (PCR) in the former. Conventional and real-time PCR failed to amplify any spp. DNA. The seroprevalence for spotted fever group/transitional group spp. in Western Australian dogs was 17.3% (54/312), and for typhus group (TG) spp., 18.4% (57/310), with a cut-off titer of 1:128. Young dogs (≤ 2 years) from Aboriginal communities had significantly lower seropositivity to TG spp. compared with all other groups, and young Perth dogs had a significantly higher seropositivity to TG spp. than all Aboriginal community dogs.
有人声称，狗可以作为有用的哨兵，用于对包括……属物种在内的媒介传播传染病进行公共卫生监测。我们利用在默多克大学兽医医院机会性采集的153份犬类血液样本，以及从西澳大利亚州西北部原住民社区采集的156份犬类血清，来检测……属物种的暴露证据，对后者采用微量免疫荧光法（MIF）检测，对前者则同时采用MIF和聚合酶链反应（PCR）检测。常规PCR和实时PCR均未能扩增出任何……属物种的DNA。西澳大利亚州犬类中斑点热群/过渡群……属物种的血清阳性率为17.3%（54/312），斑疹伤寒群（TG）……属物种的血清阳性率为18.4%（57/310），截断滴度为1:128。与所有其他组相比，来自原住民社区的幼犬（≤2岁）对TG……属物种的血清阳性率显著较低，而珀斯的幼犬对TG……属物种的血清阳性率显著高于所有原住民社区的犬类。
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