State Key Laboratory for Agro-Biotechnology, College of Biological Science, China Agricultural University, Beijing, China.
Sci Rep. 2017 Jul 19;7(1):5814. doi: 10.1038/s41598-017-06261-5.
Currently, chronic hepatitis B virus (HBV) infection remains a serious public health problem in the world. Recombinant HBV vaccine, as a preventive strategy against HBV infection, generates high antibody level, but it is not effective to activate innate and cellular immunity for chronic HBV infection therapy. Lectins from mushroom are natural and active proteins which have been shown important biological functions. However, little is known about the immunological mechanism engaged by mushroom lectins. Here we report that, lectin from Pleurotus ostreatus (POL) stimulated innate response by activating Toll-like receptor 6 signal pathway of dendritic cells. Subsequently POL enhanced HBV specific antibody level and follicular helper T cells response which overcame HBV tolerance in transgenic mice. This study suggests a novel mechanism for POL acting on immune response and a therapeutic approach to break HBV tolerance.
目前，慢性乙型肝炎病毒（HBV）感染仍然是全球严重的公共卫生问题。重组 HBV 疫苗作为预防 HBV 感染的策略，可产生高抗体水平，但对慢性 HBV 感染的治疗无法有效激活固有和细胞免疫。蘑菇来源的凝集素是天然的、具有活性的蛋白质，具有重要的生物学功能。然而，关于蘑菇凝集素参与的免疫机制知之甚少。本研究报道，来自糙皮侧耳（Pleurotus ostreatus）的凝集素（POL）通过激活树突状细胞的 Toll 样受体 6 信号通路刺激固有免疫反应。随后，POL 增强了 HBV 特异性抗体水平和滤泡辅助 T 细胞反应，从而克服了转基因小鼠中的 HBV 耐受。该研究为 POL 作用于免疫反应的新机制以及打破 HBV 耐受的治疗方法提供了新的思路。
