Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, Maharashtra, 400076, India.
Centre for Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Simrol, Indore, Madhya Pradesh, 453552, India.
Sci Rep. 2017 Jul 19;7(1):5840. doi: 10.1038/s41598-017-06060-y.
Kidney diseases remain often undiagnosed due to inefficient screening methods available at patient's disposal. Early diagnosis and effective management of kidney problems can best be addressed by the development of biosensors for commonly occurring clinical biomarkers. Here we report the development of single fluorophore and dual fluorophore ratiometric biosensors based on alginate microspheres for pH and urea analysis in urine samples. A facile method of air driven atomization was used for developing these polymeric fluorophore and enzyme based biosensors. Ratiometric biosensors were developed using layer-by-layer coating of polyelectrolyte conjugated to reference fluorophores. Biosensing studies using these biosensors showed that samples in pathophysiological range can be measured having pH range of 4-8 and urea levels between 0-50 mM. Testing of urine samples using these biosensors showed that both pH and urea detection can be accurately performed without interference. Thus, we believe that FITC-Dextran and FITC-Dextran/RuBpy based pH and urea biosensors show a great potential to be translated as a point of care device for pH and urea biosensing in early detection and continuous monitoring of kidney diseases.
由于患者可获得的筛选方法效率低下，肾脏疾病仍然常常无法诊断。通过开发用于常见临床生物标志物的生物传感器，可以最好地解决早期诊断和有效管理肾脏问题。在这里，我们报告了基于藻酸盐微球的单荧光团和双荧光团比率型生物传感器的开发，用于尿液样本中的 pH 值和尿素分析。我们使用空气驱动雾化的简便方法来开发这些基于聚合荧光团和酶的生物传感器。通过将参考荧光团连接的聚电解质逐层涂层来开发比率型生物传感器。使用这些生物传感器进行的生物传感研究表明，可以测量生理病理范围内的样品，其 pH 值范围为 4-8，尿素水平在 0-50 mM 之间。使用这些生物传感器测试尿液样本表明，可以在没有干扰的情况下准确进行 pH 值和尿素的检测。因此，我们认为 FITC-Dextran 和 FITC-Dextran/RuBpy 基 pH 值和尿素生物传感器具有很大的潜力，可以转化为用于早期检测和连续监测肾脏疾病的 pH 值和尿素生物传感的即时护理设备。
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