Discipline of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Khandwa Road, Indore, 453552, India.
Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 40076, India.
Sci Rep. 2019 Mar 22;9(1):5029. doi: 10.1038/s41598-019-41326-7.
Near infrared (NIR) fluorophores like Pt-porphyrin along with analyte specific enzymes require co-encapsulation in biocompatible and biodegradable carriers in order to be transformed into implantable biosensors for efficient and continuous monitoring of analytes in patients. The main objective of this research is to develop natural, biodegradable, biocompatible and a novel co-encapsulated system of Pt-porphyrin encapsulated polymeric nanoparticle and nano-micro hybrid carriers. A sequential emulsification-solvent evaporation and an air driven atomization technique was used for developing above matrices and testing them for fluorescence based oxygen and glucose biosensing. The results indicate Pt-porphyrin can be efficiently encapsulated in Poly-lactic acid (PLA) nanoparticles and PLA-alginate nano-micro particles with sizes ~450 nm and 10 µm, respectively. Biosensing studies have showed a linear fluorescent response in oxygen concentrations ranging from of 0-6 mM (R = 0.992). The Oxygen sensitivity was transformed into a linear response of glucose catalytic reaction in the range of 0-10 mM (R = 0.968) with a response time of 4 minutes and a stability over 15 days. We believe that the investigated NIR fluorophores like Pt-Porphyrin based nano/nano-micro hybrid carrier systems are novel means of developing biocompatible biodegradable carriers for developing implantable glucose biosensors which can efficiently manage glucose levels in diabetes.
近红外（NIR）荧光团如 Pt-卟啉与分析物特异性酶需要共包封在生物相容性和可生物降解的载体中，才能转化为用于有效和连续监测患者分析物的植入式生物传感器。这项研究的主要目标是开发天然的、可生物降解的、生物相容的和新型的共包封系统，即 Pt-卟啉包封的聚合物纳米颗粒和纳米-微混合载体。采用顺序乳化-溶剂蒸发和空气驱动雾化技术来开发上述基质，并测试它们基于荧光的氧气和葡萄糖生物传感性能。结果表明，Pt-卟啉可以有效地包封在聚乳酸（PLA）纳米颗粒和 PLA-海藻酸钠纳米-微颗粒中，粒径分别约为 450nm 和 10μm。生物传感研究表明，在 0-6mM 的氧气浓度范围内，荧光有线性响应（R=0.992）。氧气的敏感性转化为葡萄糖催化反应在 0-10mM 范围内的线性响应（R=0.968），响应时间为 4 分钟，稳定性超过 15 天。我们相信，所研究的近红外荧光团如 Pt-卟啉基纳米/纳米-微混合载体系统是开发用于开发植入式葡萄糖生物传感器的生物相容性可生物降解载体的新方法，该传感器可以有效地控制糖尿病患者的血糖水平。
