干涉旋转点扩散函数。

Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai, 201800, China.

University of Chinese Academy of Sciences, Beijing, 100049, China.

Sci Rep. 2017 Jul 19;7(1):5882. doi: 10.1038/s41598-017-06203-1.

Rotating point spread functions (PSF), such as the double helix (DH) PSF, are widely used in localization-based super-resolution imaging because of their large working depth range. In this article, we propose an interferometric DH PSF (iDH PSF) using two opposed objective lenses as in the 4Pi microscope. In the proposed iDH PSF, the super-resolution in the axial PSF is transferred to the azimuthal rotation. Moreover, we design an iDH PSF whose imaging range reaches 3 μm, which is roughly 3 times as much as that which can be obtained by using other interferometric localization-based super-resolution imaging methods.

旋转点扩散函数（PSF），如双螺旋（DH）PSF，由于其大的工作深度范围，被广泛应用于基于定位的超分辨率成像中。在本文中，我们提出了一种使用两个相对物镜的干涉双螺旋 PSF（iDH PSF），类似于 4Pi 显微镜。在提出的 iDH PSF 中，轴向 PSF 的超分辨率被转移到了角向旋转。此外，我们设计了一个 iDH PSF，其成像范围达到 3 μm，大约是其他基于干涉定位的超分辨率成像方法所能获得的成像范围的 3 倍。