血液透析患者载脂蛋白E基因多态性及血脂水平检测

Detection of Apolipoprotein E Gene Polymorphism and Blood Lipid Level in Hemodialysis Patients.

作者信息

Wang Yuxin, Wang Ning, Lu Yuanshan, Yu Qing, Zhou Lina, Xu Qinjun

机构信息

Department of Nephrology, The No.2 Hospital of Xiamen, Xiamen Medical College, Xiamen 361021, China.

The Medical College of Xiamen University, Xiamen 361000, China.

出版信息

J Clin Med Res. 2017 Aug;9(8):695-700. doi: 10.14740/jocmr3046e. Epub 2017 Jul 1.

DOI:10.14740/jocmr3046e

PMID:28725318

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5505306/

Abstract

BACKGROUND

The aim was to investigate the clinical characteristics of lipid metabolism and the effect of apolipoprotein E (ApoE) gene polymorphism on lipid metabolism in hemodialysis patients.

METHODS

The serum levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDLC), low-density lipoprotein cholesterol (LDLC), ApoA1, ApoB, ApoE and lipoprotein Lp(a) were detected by polymerase chain reaction-restriction fragment length (PCR-RFLP).

RESULTS

The level of serum TG was significantly increased and the level of HDLC was significantly decreased in hemodialysis patients. Serum TG level was 33% higher than normal, and HDLC was 10.4% less than normal. The correlation analysis showed that TG level was significantly correlated with serum albumin level and extracorporeal circulation blood flow during dialysis. HDLC was significantly correlated with KT/V. The incidence of hypertension in hemodialysis patients was 73.6% and cardiovascular disease was 25%. The level of TG in the cardiovascular disease group was significantly higher than that in the non-cardiovascular disease group, and there was no significant difference between the hypertensive group and the non-hypertensive group. ApoE gene polymorphism test showed that the frequency of ApoE genotype ε3/3 and allele ε3 was the highest in hemodialysis patients, and the levels of TC, TG and LDLC were higher in ApoE genotype ε3/4 + ε4/4.

CONCLUSION

The levels of serum TG and ApoB were significantly increased in patients with hemodialysis, and HDLC and other indexes were significantly decreased. The level of TG in patients with cardiovascular complications was significantly higher than in patients without complications. TG level was significantly correlated with serum albumin level and extracorporeal circulation blood flow during dialysis. HDLC was significantly correlated with KT/V. Hemodialysis patients who had ApoE allele ε4 are prone to lipid metabolism disorders.

摘要

背景

旨在研究血液透析患者脂质代谢的临床特征以及载脂蛋白E（ApoE）基因多态性对脂质代谢的影响。

方法

采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDLC）、低密度脂蛋白胆固醇（LDLC）、载脂蛋白A1、载脂蛋白B、载脂蛋白E和脂蛋白Lp(a)水平。

结果

血液透析患者血清TG水平显著升高，HDLC水平显著降低。血清TG水平比正常高33%，HDLC比正常低10.4%。相关性分析显示，TG水平与透析期间血清白蛋白水平及体外循环血流量显著相关。HDLC与KT/V显著相关。血液透析患者高血压发病率为73.6%，心血管疾病发病率为25%。心血管疾病组TG水平显著高于非心血管疾病组，高血压组与非高血压组之间无显著差异。ApoE基因多态性检测显示，血液透析患者中ApoE基因型ε3/3及等位基因ε3频率最高，ApoE基因型ε3/4 + ε4/4的TC、TG和LDLC水平较高。