Hanh Nguyen Thi Hong, Nhung Bui Thi, Dao Duong Thi Anh, Tuyet Le Thi, Hop Le Thi, Binh Tran Quang, Thuc Vu Thi Minh
Hanoi National University of Education, 136 Xuan Thuy Street, Hanoi, Vietnam.
National Institute of Nutrition, 48B Tang Bat Ho Street, Hanoi, Vietnam.
Lipids Health Dis. 2016 Oct 10;15(1):176. doi: 10.1186/s12944-016-0349-6.
The dyslipidemia associated with obesity plays a major role in the development of atherosclerosis and cardiovascular disease. Dyslipidemia in childhood can progress in adult stage. APOE is one of the most important genes that regulate plasma lipid transport and clearance. The study aimed to assess whether the common APOE polymorphism is associated with lipid profiles and dyslipidemia, and it could be modulated by obesity-related traits (body mass index, waist circumference, hip circumference, and waist-to-hip ratio) in Vietnamese children.
A case-control study was designed including 249 cases with dyslipidemia and 600 controls without dyslipidemia. Dyslipidemia is defined as elevated total or low-density lipoprotein (LDL) cholesterol levels, or low levels of high-density lipoprotein (HDL) cholesterol. Genotype for APOE polymorphism (rs7412 and rs429358) was determined by the polymerase chain reaction and restriction fragment length polymorphism method. The association of APOE genotypes with plasma lipid disorders was tested by binary logistic regression analysis, taking into account the confounding factors of age, sex, residence, province and obesity-related traits.
In comparison with ε3/ε3 carriers, the ε4 carriers had the highest concentration of serum TC and LDL-C in cases and controls (P ≤ 0.001), while ε2 carriers had the lowest. Carriers without TT haplotype had higher serum TC than those with TT haplotype. The ε4 carriers had higher hypoalphalipoproteinemia risk than ε3/ε3 carriers (OR = 2.78, P = 0.02) before and after adjustment for age, gender, residence and obesity-related traits.
The study suggested that the APOE genotype and haplotype significantly associated with plasma TC and LDL-C level in Vietnamese children. The association of APOE genotype with hypoalphalipoproteinemia was independent of obesity-related traits.
与肥胖相关的血脂异常在动脉粥样硬化和心血管疾病的发生发展中起主要作用。儿童期血脂异常可延续至成年期。载脂蛋白E（APOE）是调节血浆脂质转运和清除的最重要基因之一。本研究旨在评估常见的APOE基因多态性是否与血脂谱及血脂异常相关，以及它是否会受到越南儿童肥胖相关特征（体重指数、腰围、臀围和腰臀比）的影响。
设计了一项病例对照研究，纳入249例血脂异常患者和600例无血脂异常的对照。血脂异常定义为总胆固醇或低密度脂蛋白（LDL）胆固醇水平升高，或高密度脂蛋白（HDL）胆固醇水平降低。采用聚合酶链反应和限制性片段长度多态性方法测定APOE基因多态性（rs7412和rs429358）的基因型。通过二元逻辑回归分析检验APOE基因型与血浆脂质紊乱的相关性，同时考虑年龄、性别、居住地、省份和肥胖相关特征等混杂因素。
与ε3/ε3携带者相比，ε4携带者在病例组和对照组中的血清总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）浓度最高（P≤0.001），而ε2携带者最低。无TT单倍型的携带者血清TC水平高于有TT单倍型的携带者。在调整年龄、性别、居住地和肥胖相关特征前后，ε4携带者发生低脂蛋白血症的风险均高于ε3/ε3携带者（比值比[OR]=2.78，P=0.02）。
该研究表明，APOE基因型和单倍型与越南儿童的血浆TC和LDL-C水平显著相关。APOE基因型与低脂蛋白血症的关联独立于肥胖相关特征。
