Negrín-Báez Davinia, Negrín-Báez Davinia, Rodríguez-Ramilo Silvia T, Afonso Juan M, Zamorano María J
Mar Biotechnol (NY). 2016 Feb;18(1):98-106. doi: 10.1007/s10126-015-9671-7.
Morphological abnormalities, especially skeletal deformities, are some of the most important problems affecting gilthead seabream (Sparus aurata L.) aquaculture industry. In this study, a QTL analysis for LSK complex deformity in gilthead seabream is reported. LSK complex is a severe deformity consisting of a consecutive repetition of three vertebral deformities: lordosis, scoliosis, and kyphosis. Seventy-eight offspring from six breeders from a mass-spawning were analyzed: five full-sibling families, three maternal, and two paternal half-sibling families. They had shown a significant association with the LSK complex prevalence in a previous segregation analysis. Fish were genotyped using a set of multiplex PCRs (ReMsa1-13), which includes 106 microsatellite markers. Two methods were used to perform the QTL analysis: a linear regression with the GridQTL software and a linear mixed model with the Qxpak software. A total of 18 QTL were identified. Four of them (QTLSK3, 6, 12, and 14), located in LG5, 8, 17, and 20, respectively, were the most solid ones. These QTL were significant at genome level and showed an extremely large effect (>35%) with both methods. Markers close to the identified QTL showed a strong association with phenotype. Two of these molecular markers (DId-03-T and Bt-14-F) were considered as potential linked-to-this-deformity markers. The detection of these QTL supposes a critical step in the implementation of marker-assisted selection in this species, which could decrease the incidence of this deformity and other related deformities. The identification of these QTL also represents a major step towards the study of the etiology of skeletal deformities in this species.
形态学异常，尤其是骨骼畸形，是影响金头鲷（Sparus aurata L.）养殖业的一些最重要问题。在本研究中，报道了对金头鲷LSK复合畸形的QTL分析。LSK复合畸形是一种严重畸形，由三种椎体畸形（脊柱前凸、脊柱侧凸和脊柱后凸）的连续重复组成。对来自大规模产卵的六个育种家的78个后代进行了分析：五个全同胞家系、三个母系半同胞家系和两个父系半同胞家系。在先前的分离分析中，它们与LSK复合畸形的患病率显示出显著关联。使用一组多重PCR（ReMsa1 - 13）对鱼进行基因分型，该组包括106个微卫星标记。采用两种方法进行QTL分析：使用GridQTL软件进行线性回归和使用Qxpak软件进行线性混合模型分析。共鉴定出18个QTL。其中四个（QTLSK3、6、12和14）分别位于LG5、8、17和20上，是最可靠的QTL。这两种方法在基因组水平上，这些QTL均具有显著性，并显示出极大的效应（>35%）。与鉴定出的QTL附近的标记与表型显示出强关联。其中两个分子标记（DId - 03 - T和Bt - 14 - F）被认为是与这种畸形潜在连锁的标记。这些QTL的检测是该物种实施标记辅助选择的关键步骤，这可能会降低这种畸形和其他相关畸形的发生率。这些QTL的鉴定也是朝着研究该物种骨骼畸形病因学迈出的重要一步。
