The Fish Molecular Genetics and Biotechnology Laboratory, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Department of Biology, College of Art and Sciences, Syracuse University, Syracuse, NY, 13244, USA.
Mar Biotechnol (NY). 2019 Jun;21(3):335-347. doi: 10.1007/s10126-019-09883-0. Epub 2019 Mar 20.
Motile Aeromonas septicemia (MAS) disease caused by a bacterial pathogen, Aeromonas hydrophila, is an emerging but severe disease of catfish. Genetic enhancement of disease resistance is considered to be effective to control the disease. To provide an insight into the genomic basis of MAS disease resistance, in this study, we conducted a genome-wide association study (GWAS) to identify quantitative trait loci (QTL). A total of 1820 interspecific backcross catfish of 7 families were challenged with A. hydrophila, and 382 phenotypic extremes were selected for genotyping with the catfish 690 K SNP arrays. Three QTL on linkage group (LG) 2, 26 and 29 were identified to be significantly associated with MAS resistance. Within these regions, a total of 24 genes had known functions in immunity, 10 of which were involved in NF-κB signaling pathway, suggesting the importance of NF-κB signaling pathway in MAS resistance. In addition, three suggestively significant QTL were identified on LG 11, 17, and 20. The limited numbers of QTL involved in MAS resistance suggests that marker-assisted selection may be a viable approach for catfish breeding.
由细菌病原体嗜水气单胞菌引起的游动性嗜水气单胞菌败血症（MAS）疾病是一种新兴的、严重的鲶鱼疾病。遗传增强疾病抗性被认为是控制疾病的有效方法。为了深入了解 MAS 疾病抗性的基因组基础，本研究进行了全基因组关联研究（GWAS）以鉴定数量性状位点（QTL）。总共用 1820 条 7 个家系的种间回交鲶鱼进行了 A. hydrophila 攻毒实验，并用鲶鱼 690 K SNP 芯片对 382 个表型极值进行了基因型分析。在连锁群（LG）2、26 和 29 上鉴定到与 MAS 抗性显著相关的 3 个 QTL。在这些区域内，共有 24 个具有免疫功能的已知基因，其中 10 个基因参与 NF-κB 信号通路，这表明 NF-κB 信号通路在 MAS 抗性中非常重要。此外，还在 LG 11、17 和 20 上鉴定到 3 个提示性显著 QTL。参与 MAS 抗性的 QTL 数量有限，这表明标记辅助选择可能是鲶鱼养殖的可行方法。
