Zarkasi Kamarul Zaman, Taylor Richard S, Glencross Brett D, Abell Guy C J, Tamplin Mark L, Bowman John P
School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia; Tasmanian Institute of Agriculture, Food Safety Centre, University of Tasmania, Hobart, Tasmania, Australia.
CSIRO Agriculture, Hobart, Tasmania, Australia.
Res Microbiol. 2017 Oct;168(8):751-759. doi: 10.1016/j.resmic.2017.07.003. Epub 2017 Jul 17.
In this study, microbial community dynamics were assessed within a simple in vitro model system in order to understand those changes influenced by diet. The abundance and diversity of bacteria were monitored within different treatment slurries inoculated with salmon faecal samples in order to mimic the effects of dietary variables. A total of five complete diets and two ingredients (plant meal) were tested. The total viable counts (TVCs) and sequencing data revealed that there was very clear separation between the complete diets and the plant meal treatments, suggesting a dynamic response by the allochthonous bacteria to the treatments. Automated ribosomal intergenic spacer analysis (ARISA) results showed that different diet formulations produced different patterns of fragments, with no separation between the complete diets. However, plant-based protein ingredients were clearly separated from the other treatments. 16S rRNA Illumina-based sequencing analysis showed that members of the genera Aliivibrio, Vibrio and Photobacterium became predominant for all complete diets treatments. The plant-based protein ingredient treatments only sustained weak growth of the genus Sphingomonas. In vitro based testing of diets could be a useful strategy to determine the potential impact of either complete feeds or ingredients on major fish gastrointestinal tract microbiome members.
在本研究中，为了解饮食影响的变化，在一个简单的体外模型系统中评估了微生物群落动态。在接种了鲑鱼粪便样本的不同处理浆液中监测细菌的丰度和多样性，以模拟饮食变量的影响。总共测试了五种全价饲料和两种成分（植物性饲料）。总活菌数（TVC）和测序数据显示，全价饲料和植物性饲料处理之间有非常明显的区分，表明外来细菌对处理有动态反应。自动核糖体基因间隔区分析（ARISA）结果表明，不同的饲料配方产生不同的片段模式，全价饲料之间没有区分。然而，植物性蛋白质成分与其他处理明显区分开来。基于Illumina的16S rRNA测序分析表明，对于所有全价饲料处理，发光杆菌属、弧菌属和发光细菌属的成员占主导地位。植物性蛋白质成分处理仅使鞘氨醇单胞菌属维持微弱生长。基于体外的饲料测试可能是一种有用的策略，可用于确定全价饲料或成分对主要鱼类胃肠道微生物群落成员的潜在影响。
