Reyes E, Garcia K, Wolfe J, Sandoval D
Alcohol. 1986 Mar-Apr;3(2):153-5. doi: 10.1016/0741-8329(86)90026-1.
The present study was undertaken to determine the effects of the in utero administration of phenobarbital (Pb) on gamma glutamyl transpeptidase (gamma-GTP) in rats. Female Wistar rats were bred to healthy adult males. On day 1 of gestation (evidenced by spermatozoa from vaginal smear) the pregnant rats were given 100 mg/kg phenobarbital (as the sodium salt) daily via the BioServ Liquid diet throughout gestation. Pair-fed controls were given the same volume of food as consumed by the experimental group. The offspring were sacrificed at birth and body, brain and liver weights determined. The pups born to the Pb treated mothers weighed significantly less than those born to the control mothers (4.77 and 6.60 g respectively). The brains and livers were homogenized in Tris buffered saline and assayed for gamma-GTP activity. Brain gamma-GTP activity was found to be 0.00807 u in the Pb group and 0.00670 u in the control group. We also examined the effects of chronic administration of Pb in adult rats and found no apparent effect on liver or brain gamma-GTP activity.
本研究旨在确定子宫内给予苯巴比妥（Pb）对大鼠γ-谷氨酰转肽酶（γ-GTP）的影响。将雌性Wistar大鼠与健康成年雄性大鼠交配。在妊娠第1天（通过阴道涂片发现精子证实），给妊娠大鼠在整个妊娠期通过BioServ液体饲料每日给予100mg/kg苯巴比妥（作为钠盐）。配对喂养的对照组给予与实验组消耗的相同体积的食物。在出生时处死后代并测定体重、脑重和肝重。接受Pb处理的母亲所生的幼崽体重明显低于对照组母亲所生的幼崽（分别为4.77g和6.60g）。将脑和肝在Tris缓冲盐水中匀浆并测定γ-GTP活性。发现Pb组脑γ-GTP活性为0.00807u，对照组为0.00670u。我们还研究了成年大鼠慢性给予Pb的影响，发现对肝或脑γ-GTP活性没有明显影响。
