Orci L, Glick B S, Rothman J E
Cell. 1986 Jul 18;46(2):171-84. doi: 10.1016/0092-8674(86)90734-8.
Isolated Golgi membranes incubated in the presence of ATP and a cytosolic protein fraction form a population of coated buds or vesicles from the Golgi cisternae. The coats do not have the characteristic hexagonal-pentagonal basketwork of clathrin, and do not react with anti-clathrin polyclonal antibody. The conditions that produce these apparently nonclathrin-coated buds also reconstitute protein transport between compartments of the Golgi stack. The membrane of the buds contains the glycoprotein in transit through these Golgi stacks (VSV-encoded G protein). This suggests that protein transport through the Golgi stack is mediated by a new type of coated vesicle that does not contain clathrin. The concentration of G protein in the coated buds reflects the local concentration of G protein in the cisternae, raising the possibility that the Golgi coated vesicles may be "bulk" membrane carriers.
在ATP和胞质蛋白组分存在的情况下孵育的分离高尔基体膜，会从高尔基体潴泡形成一群有被小芽或小泡。这些衣被没有网格蛋白特有的六边形 - 五边形篮状结构，并且不与抗网格蛋白多克隆抗体发生反应。产生这些明显无网格蛋白包被小芽的条件也能在高尔基体堆叠的不同区室之间重建蛋白质运输。小芽的膜含有正在通过这些高尔基体堆叠转运的糖蛋白（水泡性口炎病毒编码的G蛋白）。这表明通过高尔基体堆叠的蛋白质运输是由一种不含网格蛋白的新型有被小泡介导的。有被小芽中G蛋白的浓度反映了潴泡中G蛋白的局部浓度，这增加了高尔基体有被小泡可能是“批量”膜载体的可能性。
