Cheng Lei, Long Bing, Guo Xin-Xing, Li Li-Xin, Xu Yue, Hao Lin-Lin, Zheng Dan-Ying, Cheng Bing, Liu Xing
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, China.
Department of Ophthalmology, Shenzhen People's Hospital, Jinan University, Shenzhen 518020, Guangdong Province, China.
Int J Ophthalmol. 2017 Jul 18;10(7):1101-1105. doi: 10.18240/ijo.2017.07.13. eCollection 2017.
To determine the levels of aquaporin-1 (AQP-1) in the lens epithelial cells (LECs) of primary glaucoma and to clarify its correlation with lens thickness.
This study comprised 64 eyes of 64 patients with primary glaucoma, who were divided into 3 groups: 25 eyes of 25 patients with acute primary angle-closure glaucoma (APACG), 19 eyes of 19 patients with chronic primary angle-closure glaucoma (CPACG) and 20 eyes of 20 patients with primary open angle glaucoma (POAG). This study also included 12 eyes of 12 patients with senile cataract as controls. The levels of AQP-1 in LECs were examined by real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry. The lens thickness was measured by A-scan ultrasonography.
The AQP-1 mRNA levels of LECs were 0.84±0.27, 0.69±0.34, 0.44±0.19 and 0.51±0.21 in APACG, CPACG, POAG and senile cataract group, respectively. The levels of AQP-1m RNA were significantly higher in PACG groups compared with those in senile cataract and POAG group (all <0.05). The immunohistochemistry showed the AQP-1 expression were strong-positive in PACG groups, but weak-positive in senile cataract and POAG group. A positive correlation was found between AQP-1 mRNA levels and the lens thickness (=0.645, <0.001).
These findings show that the higher expression of AQP-1 in LECs may contribute to increased lens thickness, which might be associated with the occurrence and development of PACG.
测定原发性青光眼晶状体上皮细胞（LECs）中 aquaporin-1（AQP-1）的水平，并阐明其与晶状体厚度的相关性。
本研究纳入64例原发性青光眼患者的64只眼，分为3组：25例急性原发性闭角型青光眼（APACG）患者的25只眼、19例慢性原发性闭角型青光眼（CPACG）患者的19只眼和20例原发性开角型青光眼（POAG）患者的20只眼。本研究还纳入12例老年性白内障患者的12只眼作为对照。通过实时定量聚合酶链反应（RT-qPCR）和免疫组织化学检测LECs中AQP-1的水平。用A超超声检查测量晶状体厚度。
APACG组、CPACG组、POAG组和老年性白内障组LECs的AQP-1 mRNA水平分别为0.84±0.27、0.69±0.34、0.44±0.19和0.51±0.21。PACG组的AQP-1 mRNA水平显著高于老年性白内障组和POAG组（均P<0.05）。免疫组织化学显示，PACG组AQP-1表达为强阳性，而老年性白内障组和POAG组为弱阳性。AQP-1 mRNA水平与晶状体厚度呈正相关（r=0.645，P<0.001）。
这些结果表明，LECs中AQP-1的高表达可能导致晶状体厚度增加，这可能与PACG的发生和发展有关。
