Saffouri B, Weir G C, Bitar K N, Makhlouf G M
Am J Physiol. 1980 Jun;238(6):G495-501. doi: 10.1152/ajpgi.1980.238.6.G495.
The isolated vascularly perfused rat stomach was used to study the polarity, kinetics, and stoichiometry of gastrin and somatostatin secretion and the interaction of the two antral peptides. The secretion of gastrin (79%) and somatostatin (95%) was predominantly in the circulation. Methacholine (5 x 10(-8) to 5 x 10(-4) M) produced a biphasic dose-dependent increase in gastrin secretion. The maximal gastrin response (434 +/- 89% above basal levels; P less than 0.001) was partially inhibited by 10(-8) M atropine and completely inhibited by 10(-7) M atropine. Methacholine produced a dose-dependent inhibition of somatostatin secretion; the inhibition was blocked by atropine. An inverse relationship between the secretion of gastrin and somatostatin was noted in the basal state and during infusion of methacholine or prostaglandin E2; the latter had effects on gastrin and somatostatin secretion opposite to those of methacholine. The data, together with data reported elsewhere that somatostatin antiserum stimulates gastrin secretion in the perfused stomach, are consistent with the hypothesis that gastric somatostatin secretion exerts a continuous restraint on basal gastrin secretion and that stimulation of gastrin secretion may be mediated in part by inhibition of somatostatin secretion.
采用离体血管灌注大鼠胃来研究胃泌素和生长抑素分泌的极性、动力学和化学计量关系，以及这两种胃窦肽之间的相互作用。胃泌素（79%）和生长抑素（95%）的分泌主要进入循环系统。乙酰甲胆碱（5×10⁻⁸至5×10⁻⁴M）使胃泌素分泌呈双相剂量依赖性增加。最大胃泌素反应（比基础水平高434±89%；P＜0.001）被10⁻⁸M阿托品部分抑制，被10⁻⁷M阿托品完全抑制。乙酰甲胆碱使生长抑素分泌呈剂量依赖性抑制；该抑制作用被阿托品阻断。在基础状态以及输注乙酰甲胆碱或前列腺素E2期间，观察到胃泌素和生长抑素分泌之间呈负相关；后者对胃泌素和生长抑素分泌的影响与乙酰甲胆碱相反。这些数据，连同其他地方报道的生长抑素抗血清刺激灌注胃中胃泌素分泌的数据，与以下假设一致：胃生长抑素分泌对基础胃泌素分泌持续发挥抑制作用，胃泌素分泌的刺激可能部分由生长抑素分泌的抑制介导。
