Sharpe A H, Gaulton G N, McDade K K, Fields B N, Greene M I
J Exp Med. 1984 Oct 1;160(4):1195-205. doi: 10.1084/jem.160.4.1195.
A syngeneic monoclonal antiidiotypic antibody was generated in BALB/c mice after repeated immunization with a BALB/c monoclonal anti-reovirus hemagglutinin (HA) antibody. The resultant syngeneic monoclonal antiidiotypic antibody, in the absence of adjuvant, was found to be capable of priming both BALB/c (H-2d, Igh-1a) and C3H/Hej (H-2k, Igh-1j) mice for Lyt-1+- and Lyt-2+-dependent responses against the mammalian reovirus. By the use of intertypic reassortants and variant virus analysis, the specificity of the response was finely mapped to the neutralization domain of the viral hemagglutinin (HA). Using purified monoclonal antiidiotype, we were able to compare the potency of antiidiotype to virus in terms of induction of immunity. 8 X 10(8) protein molecules were able to prime for cellular responses to reovirus. These studies indicate that in the reovirus system, T cells and B cells share idiotypic configurations, and that antiidiotypic antibodies of the type described herein may be useful in the development of vaccines against certain viral infections.
在用BALB/c单克隆抗呼肠孤病毒血凝素（HA）抗体反复免疫BALB/c小鼠后，产生了一种同基因单克隆抗独特型抗体。结果发现，在没有佐剂的情况下，所得的同基因单克隆抗独特型抗体能够使BALB/c（H-2d，Igh-1a）和C3H/Hej（H-2k，Igh-1j）小鼠对呼肠孤病毒产生依赖Lyt-1 +和Lyt-2 +的反应。通过使用异型重配体和变异病毒分析，将反应的特异性精确地定位到病毒血凝素（HA）的中和结构域。使用纯化的单克隆抗独特型抗体，我们能够在诱导免疫方面比较抗独特型抗体与病毒的效力。8×10（8）个蛋白质分子能够引发对呼肠孤病毒的细胞反应。这些研究表明，在呼肠孤病毒系统中，T细胞和B细胞共享独特型构型，并且本文所述类型的抗独特型抗体可能有助于开发针对某些病毒感染的疫苗。
