Bouaoudia-Madi Nadia, Boulekbache-Makhlouf Lila, Kadri Nabil, Dahmoune Farid, Remini Hocine, Dairi Sofiane, Oukhmanou-Bensidhoum Sonia, Madani Khodir
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J Complement Integr Med. 2017 Jun 10;14(4):/j/jcim.2017.14.issue-4/jcim-2016-0098/jcim-2016-0098.xml. doi: 10.1515/jcim-2016-0098.
Background Myrtle (Myrtus communis L) may constitute an interesting dietary source of health protective compounds. Microwave-assisted extraction (MAE) of total phenolic compounds (TPC) from myrtle leaf, stems, pericarp, and seeds was studied and the results were compared with those of the conventional method extraction (CME) in terms of extraction time. Methods Extraction yield/efficiency and antioxidant activity were measured using radical scavenging assay (DPPH•) and reducing power. Results The results show that the MAE was higher in terms of saving energy, extraction time (62 s) and extraction efficiency of bioactive compound compared to CME (2 h). Leaf presented the optimum content of total phenols (250 mg GAE.g-1 DW) and flavonoids (13.65 mg GAE.g-1 DW). However, the anthocyanin content was most important in pericarp extract (176.50±2.17 mg Cyd-3-glu g-1 DW). The antioxidant activity was important in all parts, mainly in leaves. The results indicated that appropriate microwave treatment could be an efficient process to phenolic compounds recovery and thus, better the antioxidant activity of myrtle extract. Conclusions Principal component analysis (PCA) applied to the experimental data shows that the distribution of the myrtle phenolic compounds depended on their plant part localization as well as the extraction method.
背景 香桃木（Myrtus communis L）可能是一种有趣的健康保护化合物饮食来源。本研究对香桃木叶片、茎、果皮和种子中总酚化合物（TPC）的微波辅助萃取（MAE）进行了研究，并在萃取时间方面将结果与传统方法萃取（CME）的结果进行了比较。方法 使用自由基清除试验（DPPH•）和还原能力测定萃取产率/效率和抗氧化活性。结果 结果表明，与CME（2小时）相比，MAE在节能、萃取时间（62秒）和生物活性化合物萃取效率方面更高。叶片中总酚（250 mg GAE·g-1 DW）和黄酮类化合物（13.65 mg GAE·g-1 DW）含量最高。然而，花青素含量在果皮提取物中最为重要（176.50±2.17 mg Cyd-3-glu g-1 DW）。所有部位均具有重要的抗氧化活性，主要是叶片。结果表明，适当的微波处理可能是回收酚类化合物的有效方法，从而提高香桃木提取物的抗氧化活性。结论 对实验数据进行的主成分分析（PCA）表明，香桃木酚类化合物的分布取决于其植物部位定位以及萃取方法。
