开发从桃金娘（L.）中提取生物活性化合物的新型分析微波辅助提取方法。
Development of New Analytical Microwave-Assisted Extraction Methods for Bioactive Compounds from Myrtle ( L.).
机构信息
Department of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Agrifood Campus of International Excellence (ceiA3), IVAGRO, Puerto Real, 11510 Cadiz, Spain.
Department of Chemical Engineering and Food Technology, Faculty of Sciences, University of Cadiz, Agrifood Campus of International Excellence (ceiA3), IVAGRO, Puerto Real, 11510 Cadiz, Spain.
出版信息
Molecules. 2018 Nov 16;23(11):2992. doi: 10.3390/molecules23112992.
The phenolic compounds and anthocyanins present in myrtle berries are responsible for its beneficial health properties. In the present study, a new, microwave-assisted extraction for the analysis of both phenolic compounds and anthocyanins from myrtle pulp has been developed. Different extraction variables, including methanol composition, pH, temperature, and sample⁻solvent ratio were optimized by applying a Box⁻Behnken design and response surface methodology. Methanol composition and pH were the most influential variables for the total phenolic compounds (58.20% of the solvent in water at pH 2), and methanol composition and temperature for anthocyanins (50.4% of solvent at 50 °C). The methods developed showed high repeatability and intermediate precision (RSD < 5%). Both methods were applied to myrtle berries collected in two different areas of the province of Cadiz (Spain). Hierarchical clustering analysis results show that the concentration of bioactive compounds in myrtle is related to their geographical origin.
桃金娘浆果中的酚类化合物和花青素是其有益健康特性的原因。在本研究中，开发了一种新的微波辅助提取方法，用于分析桃金娘浆中的酚类化合物和花青素。通过应用 Box⁻Behnken 设计和响应面法，优化了包括甲醇组成、pH 值、温度和样品⁻溶剂比在内的不同提取变量。甲醇组成和 pH 值是对总酚类化合物（在 pH 2 下水中 58.20%的溶剂）最具影响力的变量，而甲醇组成和温度则对花青素（50 °C 时 50.4%的溶剂）最具影响力。所开发的方法显示出较高的重复性和中间精密度（RSD<5%）。两种方法均应用于在西班牙加的斯省两个不同地区采集的桃金娘浆果。层次聚类分析结果表明，桃金娘中生物活性化合物的浓度与其地理起源有关。
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