a Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
b Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Genome. 2017 Oct;60(10):797-814. doi: 10.1139/gen-2017-0059. Epub 2017 Jul 21.
R2R3-MYB transcription factors (TFs) have been shown to play important roles in plants, including in development and in various stress conditions. Phylogenetic analysis showed the presence of 249 R2R3-MYB TFs in Brassica napus, called BnaR2R3-MYB TFs, clustered into 38 clades. BnaR2R3-MYB TFs were distributed on 19 chromosomes of B. napus. Sixteen gene clusters were identified. BnaR2R3-MYB TFs were characterized by motif prediction, gene structure analysis, and gene ontology. Evolutionary analysis revealed that BnaR2R3-MYB TFs are mainly formed as a result of whole-genome duplication. Orthologs and paralogs of BnaR2R3-MYB TFs were identified in B. napus, B. rapa, B. oleracea, and Arabidopsis thaliana using synteny-based methods. Purifying selection was pervasive within R2R3-MYB TFs. K/K values lower than 0.3 indicated that BnaR2R3-MYB TFs are being functionally converged. The role of gene conversion in the formation of BnaR2R3-MYB TFs was significant. Cis-regulatory elements in the upstream regions of BnaR2R3-MYB genes, miRNA targeting BnaR2R3MYB TFs, and post translational modifications were identified. Digital expression data revealed that BnaR2R3-MYB genes were highly expressed in the roots and under high salinity treatment after 24 h. BnaMYB21, BnaMYB141, and BnaMYB148 have been suggested for improving salt-tolerant B. napus. BnaR2R3-MYB genes were mostly up regulated on the 14th day post inoculation with Leptosphaeria biglobosa and L. maculan. BnaMYB150 is a candidate for increased tolerance to Leptospheria in B. napus.
R2R3-MYB 转录因子 (TFs) 在植物中发挥着重要作用，包括在发育过程中和各种胁迫条件下。系统发育分析表明，油菜中存在 249 个 R2R3-MYB TFs，称为 BnaR2R3-MYB TFs，分为 38 个分支。BnaR2R3-MYB TFs 分布在油菜的 19 条染色体上。鉴定出 16 个基因簇。通过基序预测、基因结构分析和基因本体论对 BnaR2R3-MYB TFs 进行了特征描述。进化分析表明，BnaR2R3-MYB TFs 主要是由于全基因组复制形成的。利用基于同线性的方法，在油菜、白菜、甘蓝和拟南芥中鉴定出 BnaR2R3-MYB TFs 的同源物和同系物。在 R2R3-MYB TFs 中普遍存在纯化选择。K/K 值低于 0.3 表明 BnaR2R3-MYB TFs 的功能正在趋同。基因转换在 BnaR2R3-MYB TFs 的形成中起着重要作用。鉴定了 BnaR2R3-MYB 基因上游区的顺式调控元件、靶向 BnaR2R3-MYB TFs 的 miRNA 以及翻译后修饰。数字表达数据显示，BnaR2R3-MYB 基因在根中高度表达，在 24 小时后高盐处理下表达上调。BnaMYB21、BnaMYB141 和 BnaMYB148 被建议用于提高油菜的耐盐性。在接种黑胫病菌和斑枯病菌 14 天后，BnaR2R3-MYB 基因大多上调。BnaMYB150 是提高油菜对黑胫病菌耐受力的候选基因。
