Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Ministry of Agriculture, Wuhan 430062, China.
Int J Mol Sci. 2023 May 30;24(11):9514. doi: 10.3390/ijms24119514.
Rapeseed ( L.) is an important crop for edible oil, vegetables, and biofuel. Rapeseed growth and development require a minimum temperature of ~1-3 °C. Notably, frost damage occurs during overwintering, posing a serious threat to the productivity and yield of rapeseed. MYB proteins are important transcription factors (TFs) in plants, and have been proven to be involved in the regulation of stress responses. However, the roles of the MYB TFs in rapeseed under cold stress conditions are yet to be fully elucidated. To better understand the molecular mechanisms of one MYB-like 17 gene, , in response to low temperature, the present study found that the transcript level of is induced by cold stress. To characterize the gene's function, the 591 bp coding sequence (CDS) from rapeseed was isolated and stably transformed into rapeseed. The further functional analysis revealed significant sensitivity in overexpression lines (-OE) after freezing stress, suggesting its involvement in freezing response. A total of 14,298 differentially expressed genes relative to freezing response were found based on transcriptomic analysis of -OE. Overall, 1321 candidate target genes were identified based on differential expression, including Phospholipases C1 (), FCS-like zinc finger 8 (), and Kinase on the inside (). The qPCR results confirmed that the expression levels of certain genes showed fold changes ranging from two to six when compared between -OE and WT lines after exposure to freezing stress. Furthermore, verification indicated that affects the promoter of , and genes. In summary, the results suggest that acts as a transcriptional repressor in regulating certain genes related to growth and development during freezing stress. These findings provide valuable genetic and theoretical targets for molecular breeding to enhance freezing tolerance in rapeseed.
油菜（ L.）是一种重要的食用油、蔬菜和生物燃料作物。油菜的生长和发育需要最低温度约为 1-3°C。值得注意的是，在越冬期间会发生霜害，这对油菜的生产力和产量构成严重威胁。MYB 蛋白是植物中重要的转录因子（TFs），已被证明参与了胁迫反应的调节。然而，MYB TFs 在油菜冷胁迫条件下的作用尚未完全阐明。为了更好地理解油菜中一个类似 MYB 的 17 号基因的分子机制，在本研究中发现 对低温胁迫的转录水平。为了表征该基因的功能，从油菜中分离并稳定转化了该基因的 591 bp 编码序列（CDS）。进一步的功能分析表明，在冷胁迫后过表达株系（-OE）中对低温敏感，表明其参与了冻害响应。基于对-OE 的转录组分析，发现了与冻害响应相关的 14298 个差异表达基因。总的来说，基于差异表达，共鉴定了 1321 个候选靶基因，包括磷脂酶 C1（）、FCS 样锌指 8（）和激酶在内部（）。qPCR 结果证实，在暴露于冷胁迫后，-OE 和 WT 系之间某些基因的表达水平显示出 2 到 6 倍的变化。此外，验证表明 影响 、 和 基因的启动子。总之，结果表明 作为一个转录抑制子，在调节某些与冻害胁迫下生长和发育相关的基因方面发挥作用。这些发现为增强油菜的抗冻性提供了有价值的遗传和理论靶标。
