非同源 MCM 亚基在 DNA 复制许可中的独特作用。
Unique Roles of the Non-identical MCM Subunits in DNA Replication Licensing.
机构信息
Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; Institute for Advanced Study, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
State Key Laboratory of Membrane Biology, Peking-Tsinghua Center for Life Sciences, School of Life Sciences, Peking University, Beijing, China.
出版信息
Mol Cell. 2017 Jul 20;67(2):168-179. doi: 10.1016/j.molcel.2017.06.016.
A family of six homologous subunits, Mcm2, -3, -4, -5, -6, and -7, each with its own unique features, forms the catalytic core of the eukaryotic replicative helicase. The necessity of six similar but non-identical subunits has been a mystery since its initial discovery. Recent cryo-EM structures of the Mcm2-7 (MCM) double hexamer, its precursors, and the origin recognition complex (ORC)-Cdc6-Cdt1-Mcm2-7 (OCCM) intermediate showed that each of these subunits plays a distinct role in orchestrating the assembly of the pre-replication complex (pre-RC) by ORC-Cdc6 and Cdt1.
一个由六个同源亚基组成的家族，Mcm2、-3、-4、-5、-6 和 -7，每个亚基都有其独特的特征，构成了真核复制解旋酶的催化核心。自最初发现以来，六个相似但不相同的亚基的必要性一直是个谜。最近的冷冻电镜结构显示，Mcm2-7（MCM）双六聚体、其前体以及起始识别复合物（ORC）-Cdc6-Cdt1-Mcm2-7（OCCM）中间物，这些亚基中的每一个都在通过 ORC-Cdc6 和 Cdt1 组装复制前复合物（pre-RC）中发挥独特的作用。
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