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A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout.
Mol Cell. 2017 Jul 20;67(2):348-354.e4. doi: 10.1016/j.molcel.2017.06.030.
2
Efficient gene targeting in golden Syrian hamsters by the CRISPR/Cas9 system.
PLoS One. 2014 Oct 9;9(10):e109755. doi: 10.1371/journal.pone.0109755. eCollection 2014.
3
Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity.
Mol Cell. 2016 Aug 4;63(3):355-70. doi: 10.1016/j.molcel.2016.07.004.
4
Synthesis of an arrayed sgRNA library targeting the human genome.
Sci Rep. 2015 Oct 8;5:14987. doi: 10.1038/srep14987.
5
Generation of an arrayed CRISPR-Cas9 library targeting epigenetic regulators: from high-content screens to in vivo assays.
Epigenetics. 2017;12(12):1065-1075. doi: 10.1080/15592294.2017.1395121. Epub 2018 Jan 12.
6
New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.
Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21.
10
CRISPR/Cas9-based genome editing in mice by single plasmid injection.
Methods Enzymol. 2014;546:319-36. doi: 10.1016/B978-0-12-801185-0.00015-5.

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1
Ontogeny Dictates Oncogenic Potential, Lineage Hierarchy, and Therapy Response in Pediatric Leukemia.
bioRxiv. 2025 Mar 20:2025.03.19.643917. doi: 10.1101/2025.03.19.643917.
3
Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes.
Nat Biomed Eng. 2025 Jan;9(1):127-148. doi: 10.1038/s41551-024-01278-4. Epub 2024 Dec 4.
4
Systematic elucidation of genetic mechanisms underlying cholesterol uptake.
Cell Genom. 2023 Apr 21;3(5):100304. doi: 10.1016/j.xgen.2023.100304. eCollection 2023 May 10.
5
Understanding neural development and diseases using CRISPR screens in human pluripotent stem cell-derived cultures.
Front Cell Dev Biol. 2023 Apr 10;11:1158373. doi: 10.3389/fcell.2023.1158373. eCollection 2023.
6
Systematic elucidation of genetic mechanisms underlying cholesterol uptake.
bioRxiv. 2023 Jan 10:2023.01.09.500804. doi: 10.1101/2023.01.09.500804.
7
CRISPR-Cas9 Genome Editing and Rapid Selection of Cell Pools.
Curr Protoc. 2022 Dec;2(12):e624. doi: 10.1002/cpz1.624.
8
Pooled genetic screens with image-based profiling.
Mol Syst Biol. 2022 Nov;18(11):e10768. doi: 10.15252/msb.202110768.
9
Tetraspanin-5-mediated MHC class I clustering is required for optimal CD8 T cell activation.
Proc Natl Acad Sci U S A. 2022 Oct 18;119(42):e2122188119. doi: 10.1073/pnas.2122188119. Epub 2022 Oct 10.
10
CRISPR-Cas9 library screening approach for anti-cancer drug discovery: overview and perspectives.
Theranostics. 2022 Apr 11;12(7):3329-3344. doi: 10.7150/thno.71144. eCollection 2022.

本文引用的文献

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Prediction of potent shRNAs with a sequential classification algorithm.
Nat Biotechnol. 2017 Apr;35(4):350-353. doi: 10.1038/nbt.3807. Epub 2017 Mar 6.
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Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
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Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.
Nat Biotechnol. 2016 Feb;34(2):184-191. doi: 10.1038/nbt.3437. Epub 2016 Jan 18.
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Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries.
Nat Commun. 2015 Aug 17;6:8083. doi: 10.1038/ncomms9083.
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Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach.
Nat Methods. 2015 Sep;12(9):823-6. doi: 10.1038/nmeth.3473. Epub 2015 Jul 13.
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Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains.
Nat Biotechnol. 2015 Jun;33(6):661-7. doi: 10.1038/nbt.3235. Epub 2015 May 11.
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A computational algorithm to predict shRNA potency.
Mol Cell. 2014 Dec 18;56(6):796-807. doi: 10.1016/j.molcel.2014.10.025. Epub 2014 Nov 26.
8
Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.
Nat Biotechnol. 2014 Dec;32(12):1262-7. doi: 10.1038/nbt.3026. Epub 2014 Sep 3.
9
Improved vectors and genome-wide libraries for CRISPR screening.
Nat Methods. 2014 Aug;11(8):783-784. doi: 10.1038/nmeth.3047.
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Microhomology-based choice of Cas9 nuclease target sites.
Nat Methods. 2014 Jul;11(7):705-6. doi: 10.1038/nmeth.3015.

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