Micro System Technology Laboratory, CSIR - Central Mechanical Engineering Research Institute, Durgapur 713209, India; Department of Biotechnology, National Institute of Technology Durgapur, Durgapur 713204, India.
Micro System Technology Laboratory, CSIR - Central Mechanical Engineering Research Institute, Durgapur 713209, India.
J Colloid Interface Sci. 2017 Nov 15;506:126-134. doi: 10.1016/j.jcis.2017.07.023. Epub 2017 Jul 8.
A new method is reported for fabrication of polymeric micro- and nanoparticles from an intermediate patterned surface originated by dewetting of a polymeric thin film. Poly (d, l-lactide-co-glycolide) or PLGA, a biocompatible polymer is used to develop a thin film over a clean glass substrate which dewets spontaneously in the micro-/nano-patterned surface of size range 50nm to 3.5µm. Since another water-soluble polymer, poly vinyl alcohol (PVA) is coated on the same glass substrate before PLGA thin film formation, developed micro-/nano-patterns are easily extracted in water in the form of micro- and nanoparticle mixture of size range 50nm to 3.0µm. This simplified method is also used to effectively encapsulate a dye molecule, rhodamine B inside the PLGA micro-/nanoparticles. The developed dye-encapsulated nanoparticles, PLGA-rhodamine are separated from the mixture and tested for in-vitro delivery application of external molecules inside human lung cancer cells. For the first time, the use of thin film dewetting technique is reported as a potential route for the synthesis of polymeric micro-/nanoparticles and effective encapsulation of external species therein.
一种新的方法用于制造聚合物微球和纳米球，其源于聚合物薄膜的去湿作用产生的中间图案化表面。聚（丙交酯-共-乙交酯）或 PLGA 是一种生物相容性聚合物，用于在清洁的玻璃基底上形成薄膜，该薄膜在尺寸范围为 50nm 至 3.5µm 的微/纳米图案化表面上自发去湿。由于在 PLGA 薄膜形成之前在相同的玻璃基底上涂覆了另一种水溶性聚合物聚乙烯醇（PVA），因此以微/纳米颗粒混合物的形式很容易在水中提取出尺寸范围为 50nm 至 3.0µm 的微/纳米图案化。这种简化的方法还用于有效地将染料分子罗丹明 B 封装在 PLGA 微/纳米颗粒内。所开发的包封染料的纳米颗粒 PLGA-罗丹明从混合物中分离出来，并测试其在体外将外部分子递送到人肺癌细胞内的应用。这是首次报道使用薄膜去湿技术作为合成聚合物微/纳米颗粒的潜在途径，并有效地将外部物质封装在其中。
