Namdari Haideh, Ghayedi Mojgan, Hadjati Jamshid, Rezaei Farhad, Kalantar Kurosh, Rahimzadeh Parisa, Salehi Eisa
Immunology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran AND Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran.
Immunology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2017 Jun;16(3):235-244.
Regulatory T cells (Tregs) are important components of the immune system that modulate responses of other cells. These cells are involved in peripheral tolerance mechanisms, so defect in development and function of these cells can result in autoimmune disease. Increasing evidence supports the role of microRNAs-21 (miR-21) in the regulation of forkhead box P3 (Foxp3) expression in Tregs. We aimed to determine whether miR-21 transfection to naive CD4+ T cells can be useful in generation of iTregs in-vitro. We investigated in-vitro differentiation of miR-21-transfected naive CD4+ T cells to iTregs and compared these iTregs to cytokine-differentiated iTregs and control group. We showed that expression of Foxp3, transforming growth factor beta (TGF-β), and interleukin-10 (IL-10) are increased in iTregs generated after miR-21 transfection in comparison with cytokine-differentiated iTregs and control group. Our findings demonstrate that miR-21 has positive role in in-vitro generation of induced regulatory T-cells (iTregs).
调节性T细胞（Tregs）是免疫系统的重要组成部分，可调节其他细胞的反应。这些细胞参与外周耐受机制，因此这些细胞的发育和功能缺陷可导致自身免疫性疾病。越来越多的证据支持微小RNA-21（miR-21）在调节性T细胞中叉头框蛋白P3（Foxp3）表达的作用。我们旨在确定将miR-21转染至初始CD4+T细胞是否有助于在体外生成诱导性调节性T细胞（iTregs）。我们研究了miR-21转染的初始CD4+T细胞向iTregs的体外分化情况，并将这些iTregs与细胞因子分化的iTregs及对照组进行比较。我们发现，与细胞因子分化的iTregs和对照组相比，miR-21转染后生成的iTregs中Foxp3、转化生长因子β（TGF-β）和白细胞介素-10（IL-10）的表达增加。我们的研究结果表明，miR-21在体外诱导调节性T细胞（iTregs）的生成中具有积极作用。
