Yang Xi, Xiao Xuan, Wu Wenyan, Shen Xuemin, Zhou Zengtong, Liu Wei, Shi Linjun
Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Oral Biomedical Engineering Laboratory, Shanghai Stomatological Hospital, Fudan University, Shanghai, China.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2017 Sep;124(3):280-285. doi: 10.1016/j.oooo.2017.05.509. Epub 2017 Jul 18.
To quantitatively examine the DNA content and nuclear morphometric status of oral leukoplakia (OL) and investigate its association with the degree of dysplasia in a cytologic study.
Oral cytobrush biopsy was carried out to obtain exfoliative epithelial cells from lesions before scalpel biopsy at the same location in a blinded series of 70 patients with OL. Analysis of nuclear morphometry and DNA content status using image cytometry was performed with oral smears stained with the Feulgen-thionin method.
Nuclear morphometric analysis revealed significant differences in DNA content amount, DNA index, nuclear area, nuclear radius, nuclear intensity, sphericity, entropy, and fractal dimension (all P < .01) between low-grade and high-grade dysplasia. DNA content analysis identified 34 patients with OL (48.6%) with DNA content abnormality. Nonhomogeneous lesion (P = .018) and high-grade dysplasia (P = .008) were significantly associated with abnormal DNA content. Importantly, the positive correlation between the degree of oral dysplasia and DNA content status was significant (P = .004, correlation coefficient = 0.342).
Cytology analysis of DNA content and nuclear morphometric status using image cytometry may support their use as a screening and monitoring tool for OL progression.
在一项细胞学研究中，定量检测口腔白斑（OL）的DNA含量和核形态计量学状态，并研究其与发育异常程度的相关性。
对70例OL患者进行盲法研究，在同一部位进行手术刀活检前，先用口腔细胞刷活检获取病变部位的脱落上皮细胞。使用图像细胞术对经福尔根-硫堇法染色的口腔涂片进行核形态计量学和DNA含量状态分析。
核形态计量学分析显示，低级别和高级别发育异常之间在DNA含量、DNA指数、核面积、核半径、核强度、球形度、熵和分形维数方面存在显著差异（均P < .01）。DNA含量分析确定34例OL患者（48.6%）存在DNA含量异常。病变不均匀（P = .018）和高级别发育异常（P = .008）与DNA含量异常显著相关。重要的是，口腔发育异常程度与DNA含量状态之间的正相关具有显著性（P = .004，相关系数 = 0.342）。
使用图像细胞术对DNA含量和核形态计量学状态进行细胞学分析，可能支持将其用作OL进展的筛查和监测工具。
