Department of Molecular and Environmental Biotechnology, Falcuty of Biology and Biotechnology, Viet Nam.
Department of Physical Chemistry, Faculty of Chemistry, University of Science, Vietnam National University, Ho Chi Minh City, Viet Nam.
Carbohydr Polym. 2017 Oct 1;173:114-120. doi: 10.1016/j.carbpol.2017.05.080. Epub 2017 May 30.
Fibroblast growth factor 2 (FGF-2) is a multi-functional protein involving in wound healing. However, FGF-2 is easily degradable in vivo, which limited its use for wound treatment. In this study, we investigated a drug-delivery model for FGF-2 via incorporation with biodegradable carboxylmethyl chitosan (CMCS). CMCS nanoparticles (NPs) could be synthesized by ionic gelation using CaCl as a crosslinking reagent with the CMCS:CaCl ratio of 1:0.8. Synthesized CMCS NPs had a diameter of 32.68±6.83nm, and were non-toxic at concentrations up to 2.5mg/ml. Incorporated CMCS:FGF-2 NPs had a diameter of 34.83±5.89nm and incorporation efficiency was 95%. CMCS:FGF-2 NPs released 36.36% and 58.47% of FGF-2 after 48h incubation in two different pH of 7.4 and 5.8, respectively. The incorporation and release processes did not have a significant effect on FGF-2 activity. Simultaneously, CMCS:FGF-2 NPs could protect FGF-2 from the degradation of trypsin in vitro. Our results laid the groundwork for the manufacturing of protein incorporated CMCS NPs for bio-applications.
成纤维细胞生长因子 2（FGF-2）是一种多功能蛋白，参与伤口愈合。然而，FGF-2 在体内很容易降解，这限制了其在伤口治疗中的应用。在这项研究中，我们通过与可生物降解的羧甲基壳聚糖（CMCS）结合来研究 FGF-2 的药物传递模型。CMCS 纳米颗粒（NPs）可以通过离子凝胶法用 CaCl 作为交联试剂合成，CMCS：CaCl 的比例为 1：0.8。合成的 CMCS NPs 的直径为 32.68±6.83nm，在高达 2.5mg/ml 的浓度下是无毒的。掺入 CMCS：FGF-2 NPs 的直径为 34.83±5.89nm，掺入效率为 95%。CMCS：FGF-2 NPs 在 pH 值分别为 7.4 和 5.8 的两种不同条件下孵育 48 小时后，分别释放了 36.36%和 58.47%的 FGF-2。掺入和释放过程对 FGF-2 活性没有显著影响。同时，CMCS：FGF-2 NPs 可以保护 FGF-2 免受体外胰蛋白酶的降解。我们的研究结果为制造用于生物应用的掺入蛋白的 CMCS NPs 奠定了基础。
