Department of Pharmaceutics, National Organization for Drug Control and Research (NODCAR), Giza, Egypt.
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, University of Sadat City, Egypt.
Carbohydr Polym. 2017 Oct 1;173:441-450. doi: 10.1016/j.carbpol.2017.05.098. Epub 2017 Jun 3.
In this work chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers was developed. Nanocarriers were prepared using solvent diffusion method by applying 2 factorial design. NLC4 had the highest desirability value (0.882) and therefore, it was chosen as an optimal nanocarrier. Itexhibited spherical shape with 253nm, 83.04% entrapment efficiency and a prolonged release of AND up to 48-h. Consecutively, NLC4 was incorporated in a chitosan-hyaluronic acid gel and lyophilized for 24h to obtain chitosan-hyaluronic acid/NLC4 nanocomposite sponge to enhance AND delivery to wound sites. The morphology of sponge was characterized by scanning electron microscopy. Nanocomposites showed a porosity of 56.22% with enhanced swelling. The in vivo evaluation in rats reveals that the chitosan-hyaluronic acid/NLC4 sponge enhances the wound healing with no scar and improved tissue quality. These results strongly support the possibility of using this novel chitosan-hyaluronic acid/NLC4 sponge for wound care application.
在这项工作中，开发了一种富含穿心莲内酯（AND）脂质纳米载体的壳聚糖-透明质酸复合海绵支架。纳米载体是通过应用 2 因素设计的溶剂扩散法制备的。NLC4 的理想值（0.882）最高，因此被选为最佳纳米载体。它表现出球形，粒径为 253nm，包封效率为 83.04%，AND 的释放时间延长至 48 小时。随后，将 NLC4 掺入壳聚糖-透明质酸凝胶中，并在 24 小时内冻干，以获得壳聚糖-透明质酸/NLC4 纳米复合海绵，从而增强 AND 向伤口部位的递送。通过扫描电子显微镜对海绵的形态进行了表征。纳米复合材料的孔隙率为 56.22%，具有增强的溶胀性。在大鼠体内评估表明，壳聚糖-透明质酸/NLC4 海绵可促进伤口愈合，无疤痕，改善组织质量。这些结果有力地支持了将这种新型壳聚糖-透明质酸/NLC4 海绵用于伤口护理应用的可能性。
