Anisha B S, Biswas Raja, Chennazhi K P, Jayakumar R
Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
Int J Biol Macromol. 2013 Nov;62:310-20. doi: 10.1016/j.ijbiomac.2013.09.011. Epub 2013 Sep 20.
The aim of this work was to develop an antimicrobial sponge composed of chitosan, hyaluronic acid (HA) and nano silver (nAg) as a wound dressing for diabetic foot ulcers (DFU) infected with drug resistant bacteria. nAg (5-20 nm) was prepared and characterized. The nanocomposite sponges were prepared by homogenous mixing of chitosan, HA and nAg followed by freeze drying to obtain a flexible and porous structure. The prepared sponges were characterized using SEM and FT-IR. The porosity, swelling, biodegradation and haemostatic potential of the sponges were also studied. Antibacterial activity of the prepared sponges was analysed using Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Klebsiella pneumonia. Chitosan-HA/nAg composite sponges showed potent antimicrobial property against the tested organisms. Sponges containing higher nAg (0.005%, 0.01% and 0.02%) concentrations showed antibacterial activity against MRSA. Cytotoxicity and cell attachment studies were done using human dermal fibroblast cells. The nanocomposite sponges showed a nAg concentration dependent toxicity towards fibroblast cells. Our results suggest that this nanocomposite sponges could be used as a potential material for wound dressing for DFU infected with antibiotic resistant bacteria if the optimal concentration of nAg exhibiting antibacterial action with least toxicity towards mammalian cells is identified.
这项工作的目的是开发一种由壳聚糖、透明质酸（HA）和纳米银（nAg）组成的抗菌海绵，作为感染耐药菌的糖尿病足溃疡（DFU）的伤口敷料。制备并表征了5-20纳米的nAg。通过壳聚糖、HA和nAg的均匀混合，然后冷冻干燥以获得柔性多孔结构，制备了纳米复合海绵。使用扫描电子显微镜（SEM）和傅里叶变换红外光谱（FT-IR）对制备的海绵进行了表征。还研究了海绵的孔隙率、溶胀性、生物降解性和止血潜力。使用大肠杆菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌（MRSA）、铜绿假单胞菌和肺炎克雷伯菌分析了制备海绵的抗菌活性。壳聚糖-HA/nAg复合海绵对受试微生物显示出强大的抗菌性能。含有较高nAg浓度（0.005%、0.01%和0.02%）的海绵对MRSA显示出抗菌活性。使用人皮肤成纤维细胞进行了细胞毒性和细胞附着研究。纳米复合海绵对成纤维细胞显示出nAg浓度依赖性毒性。我们的结果表明，如果确定了对哺乳动物细胞毒性最小且具有抗菌作用的nAg最佳浓度，这种纳米复合海绵可作为感染抗生素耐药菌的DFU伤口敷料的潜在材料。
