Department of Obstetrics and Gynecology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China; Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei, Anhui 230027, China.
Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei, Anhui 230027, China.
Ultrason Sonochem. 2017 Nov;39:197-207. doi: 10.1016/j.ultsonch.2017.03.019. Epub 2017 Apr 20.
Oxygen and Indocyanine Green (ICG) loaded microparticles (OI-MPs) were fabricated by a gas-driven coaxial flow focusing (CFF) process for dual-mode imaging and sonodynamic therapy (SDT). The produced OI-MPs agent showed stable optical properties, superior imaging depth in near infrared (NIR) fluorescence imaging, and enhanced acoustic contrast after ultrasound mediation. We hypothesized that encapsulating ICG and oxygen in microparticles would enhance reactive oxygen species (ROS) production in SDT. This hypothesis was validated in a cell-free environment. We further hypothesized that ultrasound mediated fragmentation of the OI-MPs would induce cytotoxicity and apoptosis of cancer cells. This hypothesis was validated in SKOV3 ovarian cancer cells. Our research demonstrated that OI-MPs can be potentially used as a dual-mode theranostic agent for image guided SDT with enhanced efficacy. Further study is needed to delineate the mechanism of ROS-induced cell apoptosis and optimize the OI-MPs formulation for the maximal anti-cancer potency.
氧和吲哚菁绿（ICG）负载的微球（OI-MPs）通过气驱同轴流聚焦（CFF）工艺制备，用于双模成像和声动力学治疗（SDT）。所制备的 OI-MPs 试剂表现出稳定的光学特性，在近红外（NIR）荧光成像中有更好的成像深度，并且在超声介导后增强了声对比度。我们假设将 ICG 和氧气封装在微球中会增强 SDT 中的活性氧（ROS）产生。这一假设在无细胞环境中得到了验证。我们进一步假设 OI-MPs 的超声介导碎裂会诱导癌细胞的细胞毒性和细胞凋亡。这一假设在 SKOV3 卵巢癌细胞中得到了验证。我们的研究表明，OI-MPs 可作为一种双模治疗剂，用于图像引导的 SDT，具有增强的疗效。需要进一步研究来阐明 ROS 诱导细胞凋亡的机制，并优化 OI-MPs 配方以获得最大的抗癌效力。
