Tang Qin, Chang Shufang, Tian Zhonghua, Sun Jiangchuan, Hao Lan, Wang Zhigang, Zhu Shenyin
Department of Pharmacy, First Affiliated Hospital of Chongqing Medical University, Chongqing, China; Department of Ultrasound, Institute of Ultrasound Imaging, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China; State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and Ministry of Science and Technology, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, China.
Department of Obstetrics and Gynecology, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Ultrasound Med Biol. 2017 Nov;43(11):2690-2698. doi: 10.1016/j.ultrasmedbio.2017.06.030. Epub 2017 Aug 2.
Sonodynamic therapy (SDT) has become a new therapeutic method because of its activation of certain sensitizers by ultrasound. Some studies have reported that indocyanine green (ICG) has the characteristics of a sonosensitizer and favorable fluorescence imaging in synovitis of early inflammatory arthritis. In this study, we aimed to investigate the cytotoxic effect of ICG-mediated SDT on MH7A cells in vitro and the potential mechanisms involved. ICG was found to be taken up mainly in cytoplasm, with maximal uptake in 4 h. Cell viability in ICG-mediated SDT (SDT-0.5 and SDT-1.0) groups decreased significantly to 73.09 ± 1.97% and 54.24 ± 4.66%, respectively; cell apoptosis increased significantly to 26.43 ± 0.91% and 45.93 ± 6.17%, respectively. Moreover, marked loss in mitochondrial membrane potential and greatly increased generation of reactive oxygen species were observed in ICG-mediated SDT groups. Interestingly, the loss in cell viability could be effectively rescued with pretreatment with the reactive oxygen species scavenger N-acetylcysteine. These results indicate that ICG-mediated SDT is cytotoxic to fibroblast-like synoviocytes and is a potential modality for targeted therapy of synovitis in rheumatoid arthritis.
声动力疗法（SDT）因其能通过超声激活某些敏化剂而成为一种新的治疗方法。一些研究报道，吲哚菁绿（ICG）具有声敏剂的特性，在早期炎症性关节炎滑膜炎中具有良好的荧光成像效果。在本研究中，我们旨在研究ICG介导的SDT对体外MH7A细胞的细胞毒性作用及其潜在机制。发现ICG主要被摄取到细胞质中，在4小时时摄取量最大。ICG介导的SDT组（SDT-0.5和SDT-1.0）的细胞活力分别显著降低至73.09±1.97%和54.24±4.66%；细胞凋亡分别显著增加至26.43±0.91%和45.93±6.17%。此外，在ICG介导的SDT组中观察到线粒体膜电位明显丧失，活性氧生成大幅增加。有趣的是，用活性氧清除剂N-乙酰半胱氨酸预处理可以有效挽救细胞活力的丧失。这些结果表明，ICG介导的SDT对成纤维细胞样滑膜细胞具有细胞毒性，是类风湿性关节炎滑膜炎靶向治疗的一种潜在方式。
