活体多光子显微镜作为研究肾脏生理学和病理生理学的工具。
Intravital multiphoton microscopy as a tool for studying renal physiology and pathophysiology.
机构信息
Indiana University School of Medicine, Roudebush VAMC, Indiana Center for Biological Microscopy, Indianapolis, IN 46202, USA.
Indiana University School of Medicine, Roudebush VAMC, Indiana Center for Biological Microscopy, Indianapolis, IN 46202, USA.
出版信息
Methods. 2017 Sep 1;128:20-32. doi: 10.1016/j.ymeth.2017.07.014. Epub 2017 Jul 19.
Abstract
The kidney is a complex and dynamic organ with over 40 cell types, and tremendous structural and functional diversity. Intravital multi-photon microscopy, development of fluorescent probes and innovative software, have rapidly advanced the study of intracellular and intercellular processes within the kidney. Researchers can quantify the distribution, behavior, and dynamic interactions of up to four labeled chemical probes and proteins simultaneously and repeatedly in four dimensions (time), with subcellular resolution in near real time. Thus, multi-photon microscopy has greatly extended our ability to investigate cell biology intravitally, at cellular and subcellular resolutions. Therefore, the purpose of the chapter is to demonstrate how the use in intravital multi-photon microscopy has advanced the understanding of both the physiology and pathophysiology of the kidney.
摘要
肾脏是一个复杂而动态的器官，拥有超过 40 种细胞类型，具有巨大的结构和功能多样性。活体多光子显微镜、荧光探针的发展以及创新软件的应用，极大地推动了对肾脏内细胞内和细胞间过程的研究。研究人员可以同时并重复地在四个维度（时间）内定量分析多达四种标记的化学探针和蛋白质的分布、行为和动态相互作用，具有亚细胞分辨率的近实时性。因此，多光子显微镜极大地扩展了我们在活体状态下，以细胞和亚细胞分辨率研究细胞生物学的能力。因此，本章的目的是展示活体多光子显微镜的应用如何促进了对肾脏生理学和病理生理学的理解。
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