Group LEPAMAP, Department of Chemical Engineering, University of Girona, M. Aurèlia Campmany 61, 17071, Girona, Spain.
Group LEPAMAP, Department of Chemical Engineering, University of Girona, M. Aurèlia Campmany 61, 17071, Girona, Spain.
Int J Biol Macromol. 2017 Dec;105(Pt 1):741-748. doi: 10.1016/j.ijbiomac.2017.07.094. Epub 2017 Jul 19.
In this work we report the production of cellulose nanopapers modified with alkyl ketene dimer (AKD) in order to allow the immobilization of antimicrobial peptides (AMPs) to produce surfaces with antimicrobial properties. Cellulose nanofibers (CNF) were prepared from softwood bleached pulp via high pressure homogenization after chemical pretreatment of fibers via TEMPO oxidation. Nanopapers were then prepared after a casting technique and functionalized with alkyl ketene dimer before AMPs were immobilized. The immobilization process was performed by submerging the samples into AMP aqueous solutions and then dried at room temperature. Antimicrobial activity was tested against B. subtilis. Results indicated that AMPs were bound onto the nanopaper surface and released when the nanopaper was put in contact with the culture medium, which effectively demonstrates the viability of the immobilization process.
在这项工作中，我们报告了用烷基烯酮二聚体 (AKD) 改性的纤维素纳米纸的生产，以便能够固定抗菌肽 (AMPs)，从而生产具有抗菌性能的表面。通过 TEMPO 氧化对纤维进行化学预处理后，使用高压匀浆从软木漂白浆中制备纤维素纳米纤维 (CNF)。然后通过浇铸技术制备纳米纸，并在固定 AMPs 之前用烷基烯酮二聚体对其进行功能化。固定化过程是通过将样品浸入 AMP 水溶液中，然后在室温下干燥来完成的。抗菌活性测试针对枯草芽孢杆菌。结果表明，AMPs 结合到纳米纸表面上，并在纳米纸与培养基接触时释放出来，这有效地证明了固定化过程的可行性。
