Horiguchi Akio, Shinchi Masayuki, Nakamura Akiko, Wada Takatsugu, Ito Keiichi, Asano Tomohiko, Shinmoto Hiroshi, Tsuda Hitoshi, Ishihara Miya
Department of Urology, National Defense Medical College, Tokorozawa-City, Saitama, Japan.
Department of Urology, National Defense Medical College, Tokorozawa-City, Saitama, Japan.
Urology. 2017 Oct;108:212-219. doi: 10.1016/j.urology.2017.07.008. Epub 2017 Jul 19.
To investigate a link between the appearance of photoacoustic imaging (PAI) and microvasculature in prostate cancer and to assess the feasibility of PAI for angiogenesis imaging in prostate cancer.
We have developed a PAI system equipped with a transrectal ultrasound (TRUS)-type probe. Three patients who underwent PAI just before prostate biopsy and subsequently underwent radical prostatectomy were included. The PAI appearance was retrospectively reviewed, and in each patient, 4 representative areas were selected: 1 with high PAI signal intensity, 1 with low PAI signal intensity, 1 peripheral to the index tumor, and 1 inside the index tumor. The correlation of PAI intensity with 3 microvascular parameters-microvascular density, total vascular area (TVA), and total vascular length (TVL)-assessed by CD34-immunostaining in resected specimens was analyzed.
In all 3 patients the PAI intensity, TVA, and TVL in areas with high-intensity PAI signals were significantly higher than those in areas with low-intensity PAI signals, suggesting that PAI appearance describes the distribution of microvasculature in prostatic tissue correctly. All index tumors showed a ring-like PAI appearance consisting of a peripheral area of high signal intensity completely or partially surrounding an area with low signal intensity. The PAI intensity, TVA, and TVL in the periphery of the index tumors were significantly higher than those inside of the index tumors.
The intensity of PAI signals might reflect the microvascularity in normal prostatic tissues and index tumors. PAI could be a novel modality for imaging prostate cancer angiogenesis.
研究前列腺癌中光声成像（PAI）表现与微血管之间的联系，并评估PAI用于前列腺癌血管生成成像的可行性。
我们开发了一种配备经直肠超声（TRUS）型探头的PAI系统。纳入了3例在前列腺活检前接受PAI检查并随后接受根治性前列腺切除术的患者。对PAI表现进行回顾性分析，在每位患者中选择4个代表性区域：1个PAI信号强度高的区域、1个PAI信号强度低的区域、1个在索引肿瘤周边的区域和1个在索引肿瘤内部的区域。分析PAI强度与通过切除标本中CD34免疫染色评估的3个微血管参数——微血管密度、总血管面积（TVA）和总血管长度（TVL）之间的相关性。
在所有3例患者中，PAI信号强度高的区域的PAI强度、TVA和TVL均显著高于PAI信号强度低的区域，这表明PAI表现能够正确描述前列腺组织中微血管的分布。所有索引肿瘤均呈现环状PAI表现，由高信号强度的周边区域完全或部分包围低信号强度区域组成。索引肿瘤周边的PAI强度、TVA和TVL显著高于索引肿瘤内部。
PAI信号强度可能反映正常前列腺组织和索引肿瘤中的微血管情况。PAI可能是一种用于前列腺癌血管生成成像的新型方法。
