Jeton Florine, Soliz Jorge, Marchant Dominique, Joseph Vincent, Richalet Jean-Paul, Pichon Aurélien, Voituron Nicolas
Université Paris 13, Sorbonne Paris Cité, UFR SMBH, Laboratoire Hypoxie et poumons, EA 2363, 93017 Bobigny, France; Laboratory of Excellence (Labex) GR-Ex, PRES Sorbonne Paris Cité, France.
Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Quebec, QC, Canada.
Respir Physiol Neurobiol. 2017 Nov;245:98-104. doi: 10.1016/j.resp.2017.07.002. Epub 2017 Jul 19.
Previous studies suggest that chronic erythropoietin (Epo) deficiency in male mice does not alter normoxic/hypoxic ventilation. As effects of Epo are sex specific and as progesterone could be a respiratory stimulant, we evaluated the impact of Epo deficiency and its possible interaction with progesterone in ventilatory control in female mice during estrous cycle phases. Compared to wild type (WT) animals, Epo-TAg female mice exhibited higher ventilation in hypoxia. However, when data were separated into luteal and follicular phases of the estrous cycle, basal ventilation and hypoxic ventilation were not different in both mice strains. As progesterone is known to be a potent respiratory stimulant, additional experiments were performed to elucidate its role. Interestingly, after mifepristone treatment, HVR was not modified in WT and Epo-TAg mice, showing that the ventilatory stimulation observed in females was not directly mediated by progesterone. We conclude that Epo-TAg female mice show no estrous stage-dependent increase of ventilatory control and progesterone independent response to hypoxia.
先前的研究表明，雄性小鼠慢性促红细胞生成素（Epo）缺乏不会改变常氧/低氧通气。由于Epo的作用具有性别特异性，且孕酮可能是一种呼吸刺激剂，我们评估了Epo缺乏及其与孕酮在雌性小鼠发情周期各阶段通气控制中的可能相互作用。与野生型（WT）动物相比，Epo-TAg雌性小鼠在低氧时表现出更高的通气。然而，当将数据按发情周期的黄体期和卵泡期分开时，两种小鼠品系的基础通气和低氧通气并无差异。由于已知孕酮是一种有效的呼吸刺激剂，因此进行了额外的实验以阐明其作用。有趣的是，米非司酮治疗后，WT和Epo-TAg小鼠的低氧通气反应（HVR）未改变，表明在雌性小鼠中观察到的通气刺激并非直接由孕酮介导。我们得出结论，Epo-TAg雌性小鼠的通气控制没有发情阶段依赖性增加，且对低氧的反应不依赖于孕酮。
