Mondal Dibakar, Dixon S Jeffrey, Mequanint Kibret, Rizkalla Amin S
Department of Chemical and Biochemical Engineering, University of Western Ontario, London, ON, Canada N6A 5B9; Bone and Joint Institute, University of Western Ontario, London, ON, Canada N6A 5A5.
Department of Physiology and Pharmacology, University of Western Ontario, London, ON, Canada N6A 5C1; Schulich Dentistry, University of Western Ontario, London, ON, Canada N6A 5C1; Bone and Joint Institute, University of Western Ontario, London, ON, Canada N6A 5A5.
J Mech Behav Biomed Mater. 2017 Nov;75:180-189. doi: 10.1016/j.jmbbm.2017.07.010. Epub 2017 Jul 5.
Organic-inorganic class II hybrid materials have domain sizes at the molecular level and chemical bonding between the organic and inorganic phases. We have previously reported the synthesis of class II hybrid biomaterials from alkoxysilane-functionalized polycaprolactone (PCL) and borophosphosilicate (BO-PO-SiO) glass (BPSG) through a non-aqueous sol-gel process. In the present study, the mechanical properties and degradability of these PCL/BPSG hybrid biomaterials were studied and compared to those of their conventional composite counterparts. The compressive strength, modulus and toughness of the hybrid biomaterials were significantly greater compared to the conventional composites, likely due to the covalent bonding between the organic and inorganic phases. A hybrid biomaterial (50wt% PCL and 50wt% BPSG) exhibited compressive strength, modulus and toughness values of 32.2 ± 3.5MPa, 573 ± 85MPa and 1.54 ± 0.03MPa, respectively; whereas the values for composite of similar composition were 18.8 ± 1.6MPa, 275 ± 28MPa and 0.76 ± 0.03MPa, respectively. Degradation in phosphate-buffered saline was slower for hybrid biomaterials compared to their composite counterparts. Thus, these hybrid materials possess superior mechanical properties and more controlled degradation characteristics compared to their corresponding conventional composites. To assess in vitro cytocompatibility, MC3T3-E1 pre-osteoblastic cells were seeded onto the surfaces of hybrid biomaterials and polycaprolactone (control). Compared to polycaprolactone, cells on the hybrid material displayed enhanced spreading, focal adhesion formation, and cell number, consistent with excellent cytocompatibility. Thus, based on their mechanical properties, degradability and cytocompatibility, these novel biomaterials have potential for use as scaffolds in bone tissue engineering and related applications.
有机-无机II类杂化材料在分子水平上具有畴尺寸，且有机相和无机相之间存在化学键合。我们之前曾报道过通过非水溶胶-凝胶法，由烷氧基硅烷官能化的聚己内酯（PCL）和硼磷硅酸盐（BO-PO-SiO）玻璃（BPSG）合成II类杂化生物材料。在本研究中，对这些PCL/BPSG杂化生物材料的力学性能和降解性进行了研究，并与传统复合材料的性能进行了比较。与传统复合材料相比，杂化生物材料的抗压强度、模量和韧性显著更高，这可能归因于有机相和无机相之间的共价键合。一种杂化生物材料（50wt% PCL和50wt% BPSG）的抗压强度、模量和韧性值分别为32.2±3.5MPa、573±85MPa和1.54±0.03MPa；而类似组成的复合材料的值分别为18.8±1.6MPa、275±28MPa和0.76±0.03MPa。与复合材料相比，杂化生物材料在磷酸盐缓冲盐溶液中的降解较慢。因此，与相应的传统复合材料相比，这些杂化材料具有优异的力学性能和更可控的降解特性。为了评估体外细胞相容性，将MC3T3-E1前成骨细胞接种到杂化生物材料和聚己内酯（对照）的表面。与聚己内酯相比，杂化材料上的细胞表现出更强的铺展、粘着斑形成和细胞数量增加，这与优异的细胞相容性一致。因此，基于其力学性能、降解性和细胞相容性，这些新型生物材料有潜力用作骨组织工程及相关应用中的支架。
