Yaseen Yazen, Gancel Frédérique, Béchet Max, Drider Djamel, Jacques Philippe
Charles Viollette Institute, University Lille 1, Sciences and Technologies, Cité Scientifique, 59655, Villeneuve d'Ascq, France.
Terra Teaching and Research Centre, Microbial Processes and Interactions, Gembloux Agro-Bio Tech, University of Liege, Gembloux, B-5030, Liège, Belgium.
Arch Microbiol. 2017 Dec;199(10):1371-1382. doi: 10.1007/s00203-017-1406-x. Epub 2017 Jul 22.
This work aimed to rely expression of the fengycin promoter to fengycin production under different culture conditions. To this end, Bacillus subtilis BBG208, derived from BBG21, which is a fengycin overproducing strain carrying the green fluorescent protein (GFP) under the control of fengycin promoter, was used to assess the effects of different carbon and nitrogen sources on surfactin and fengycin production and the fengycin promoter expression. The data showed that some carbon sources oriented synthesis of one family of lipopeptides, while most of the nitrogen sources allowed high co-production of fengycin and surfactin. High expressions of promoter P and fengycin synthesis were obtained with urea or urea + ammonium mixture as nitrogen source and mannitol as carbon source. Moreover, temperature, pH and oxygenation influenced their biosynthesis based on the nutrition conditions. Optimization of the production medium increased the fengycin production to 768 mg L, which is the highest level reported for this strain. This study defines the suitable nutrient conditions allowing as well the highest expression of the fengycin promoter and portrays the conditions relying on the fengycin and surfactin production.
这项工作旨在研究在不同培养条件下丰原素启动子的表达与丰原素产量之间的关系。为此，使用了源自BBG21的枯草芽孢杆菌BBG208，BBG21是一株丰原素高产菌株，其携带在丰原素启动子控制下的绿色荧光蛋白（GFP），用于评估不同碳源和氮源对表面活性素和丰原素产量以及丰原素启动子表达的影响。数据表明，一些碳源有利于一类脂肽的合成，而大多数氮源能使丰原素和表面活性素大量共同产生。以尿素或尿素+铵混合物作为氮源、甘露醇作为碳源时，启动子P和丰原素合成均有高表达。此外，基于营养条件，温度、pH值和氧合作用会影响它们的生物合成。优化生产培养基后，丰原素产量提高到768 mg/L，这是该菌株报道的最高水平。本研究确定了适合的营养条件，这些条件既能使丰原素启动子实现最高表达，又描绘了丰原素和表面活性素生产所依赖的条件。
