Yaseen Yazen, Diop Awa, Gancel Frédérique, Béchet Max, Jacques Philippe, Drider Djamel
Université de Lille, INRA, Université d'Artois, Université du Littoral-Côte d'Opale, EA 7394 - ICV-Institut Charles Viollette, F-59000, Lille, France.
Arch Microbiol. 2018 Jul;200(5):783-791. doi: 10.1007/s00203-018-1483-5. Epub 2018 Feb 8.
Bacillus subtilis is a wealth source of lipopeptide molecules such as iturins, surfactins and fengycins or plipastatins endowed with a range of biological activities. These molecules, designated secondary metabolites, are synthesized via non-ribosomal peptides synthesis (NRPS) machinery and are most often subjected to a complex regulation with involvement of several regulatory factors. To gain novel insights on mechanism regulating fengycin production, we investigated the effect of the fascinating polynucleotide phosphorylase (PNPase), as well as the effect of lipopeptide surfactin. Compared to the wild type, the production of fengycin in the mutant strains B. subtilis BBG235 and BBG236 altered for PNPase has not only decreased to about 70 and 40%, respectively, but also hampered its antifungal activity towards the plant pathogen Botrytis cinerea. On the other hand, mutant strains BBG231 (srfAA) and BBG232 (srfAC) displayed different levels of fengycin production. BBG231 had registered an important decrease in fengycin production, comparable to that observed for BBG235 or BBG236. This study permitted to establish that the products of pnpA gene (PNPase), and srfAA (surfactin synthetase) are involved in fengycin production.
枯草芽孢杆菌是iturins、表面活性素、丰原素或普利帕他汀等脂肽分子的丰富来源，这些脂肽分子具有一系列生物活性。这些分子被称为次级代谢产物，通过非核糖体肽合成（NRPS）机制合成，并且通常受到多种调控因子参与的复杂调控。为了深入了解丰原素产生的调控机制，我们研究了迷人的多核苷酸磷酸化酶（PNPase）的作用以及脂肽表面活性素的作用。与野生型相比，PNPase发生改变的枯草芽孢杆菌突变株BBG235和BBG236中丰原素的产量不仅分别降至约70%和40%，而且还削弱了其对植物病原菌灰葡萄孢的抗真菌活性。另一方面，突变株BBG231（srfAA）和BBG232（srfAC）表现出不同水平的丰原素产量。BBG231的丰原素产量显著下降，与BBG235或BBG236的情况相当。这项研究证实了pnpA基因（PNPase）和srfAA（表面活性素合成酶）的产物参与了丰原素的产生。
