Department of Microbiology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Közép fasor 52., Hungary.
Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary.
Molecules. 2018 Oct 18;23(10):2675. doi: 10.3390/molecules23102675.
Surfactins are lipopeptide-type biosurfactants produced mainly by species, consisting of a peptide loop of seven amino acids and a hydrophobic fatty acid chain (C⁻C). These molecules have been proven to exhibit various biological activities; thus, their therapeutic and environmental applications are considered. Within the surfactin lipopeptide family, there is a wide spectrum of different homologues and isomers; to date, more than 30 variants have been described. Since the newest members of these lipopeptides were described recently, there is no information that is available on their characteristic features, e.g., the dependence of their production from different cultivation parameters. This study examined the effects of both the different carbon sources and various metal ions on the surfactin production of a selected strain. Among the applied carbon sources, fructose and xylose had the highest impacts on the ratio of the different variants, regarding both the peptide sequences and the lengths of the fatty acids. Furthermore, the application of metal ions Mn, Cu and Ni in the media completely changed the surfactin variant compositions of the fermenting broths leading to the appearance of methyl esterified surfactin forms, and resulted in the appearance of novel surfactin variants with fatty acid chains containing no more than 11 carbon atoms.
表面活性剂是由 种产生的脂肽型生物表面活性剂，由七个氨基酸组成的肽环和一个疏水脂肪酸链（C⁻C）组成。这些分子已被证明具有多种生物活性；因此，它们的治疗和环境应用被认为是。在表面活性剂脂肽家族中，存在着广泛的不同同源物和异构体；迄今为止，已经描述了 30 多种变体。由于这些脂肽的最新成员最近才被描述，因此，关于它们的特征，例如它们的产生是否依赖于不同的培养参数，没有任何信息。本研究考察了不同碳源和各种金属离子对选定 株菌表面活性剂产生的影响。在应用的碳源中，果糖和木糖对不同变体的比例影响最大，无论是肽序列还是脂肪酸的长度。此外，在培养基中添加金属离子 Mn、Cu 和 Ni 完全改变了发酵液中表面活性剂变体的组成，导致出现甲酯化表面活性剂形式，并产生含有不超过 11 个碳原子的脂肪酸链的新型表面活性剂变体。
