Zhang Jiaqi, Yang Xinmiao, Qiu Jiajia, Zhang Wen, Yang Jie, Han Jinzhi, Ni Li
Food Nutrition and Health Research Center, School of Advanced Manufacturing, Fuzhou University, Jinjiang, 362200, Fujian, China.
Institute of Food Science and Technology, College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108, Fujian, China.
Probiotics Antimicrob Proteins. 2025 Jun;17(3):1624-1647. doi: 10.1007/s12602-024-10447-5. Epub 2024 Dec 30.
This paper provides a comprehensive review of antimicrobial peptides (AMPs) derived from Bacillus spp. The classification and structure of Bacillus-derived AMPs encompass a diverse range. There are 89 documented Bacillus-derived AMPs, which exhibit varied sources, amino acid sequences, and molecular structures. These AMPs can be categorized into classes I, Ia, IIa, IIb, IIc, and IId. The synthesis pathway of the AMPs primarily involves either ribosomally synthesized or non-ribosomally synthesized approaches. Additionally, the antimicrobial activity of these AMPs is versatile, targeting bacteria, fungi, and viruses, through disrupting intracellular DNA and the cell wall and membrane, as well as modulating immune responses. Moreover, the Bacillus-derived AMPs demonstrate promising application in the pharmaceutical industry, environmental protection, food preservation, and bio-control in agriculture. The commonly employed strategies for enhancing the production of Bacillus-derived AMPs involve optimizing cultivation conditions, implementing systems metabolic engineering, employing genome shuffling techniques, optimizing promoters, and improving expression host optimization. This review can provide a valuable reference for comprehending the current research status on advancements and sustainable production of Bacillus-derived AMPs.
本文全面综述了来源于芽孢杆菌属的抗菌肽。芽孢杆菌属来源的抗菌肽的分类和结构多种多样。有89种已记录的芽孢杆菌属来源的抗菌肽，它们具有不同的来源、氨基酸序列和分子结构。这些抗菌肽可分为I类、Ia类、IIa类、IIb类、IIc类和IId类。抗菌肽的合成途径主要涉及核糖体合成或非核糖体合成方法。此外，这些抗菌肽的抗菌活性具有多样性，通过破坏细胞内DNA、细胞壁和细胞膜以及调节免疫反应来靶向细菌、真菌和病毒。此外，芽孢杆菌属来源的抗菌肽在制药工业、环境保护、食品保鲜和农业生物防治方面显示出有前景的应用。提高芽孢杆菌属来源抗菌肽产量的常用策略包括优化培养条件、实施系统代谢工程、采用基因组改组技术、优化启动子以及改善表达宿主优化。本综述可为理解芽孢杆菌属来源抗菌肽的进展和可持续生产的当前研究状况提供有价值的参考。
