Barua Pragya, Gayen Dipak, Lande Nilesh Vikram, Chakraborty Subhra, Chakraborty Niranjan
National Institute of Plant Genome Research, Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi, 110067, India.
Methods Mol Biol. 2017;1631:163-179. doi: 10.1007/978-1-4939-7136-7_10.
Global proteome profiling is a direct representation of the protein set in an organism, organ, tissues, or an organelle. One of the main objectives of proteomic analysis is the comparison and relative quantitation of proteins under a defined set of conditions. Two-dimensional gel electrophoresis (2-DE) has gained prominence over the last 4 decades for successfully aiding differential proteomics, providing visual confirmation of changes in protein abundance, which otherwise cannot be predicted from genome analysis. Each protein spot on 2-DE gel can be analyzed by its abundance, location, or even its presence or absence. This versatile gel-based method combines and utilizes the finest principle for separation of protein complexes by virtue of their charge and mass, visual mapping coupled with successful mass spectrometric identification of individual proteins.
全球蛋白质组分析是生物体、器官、组织或细胞器中蛋白质组的直接体现。蛋白质组学分析的主要目标之一是在一组特定条件下对蛋白质进行比较和相对定量。在过去40年里，二维凝胶电泳（2-DE）在辅助差异蛋白质组学方面成效显著，它能直观地证实蛋白质丰度的变化，而这些变化无法从基因组分析中预测得到。二维凝胶电泳上的每个蛋白质点都可以通过其丰度、位置，甚至其存在与否来进行分析。这种基于凝胶的通用方法结合并运用了根据电荷和质量分离蛋白质复合物的最佳原理，通过可视化图谱，再结合成功的质谱鉴定来识别单个蛋白质。
