Headley S A, Pretto-Giordano L G, Lima S C, Suhett W G, Pereira A H T, Freitas L A, Suphoronski S A, Oliveira T E S, Alfieri A F, Pereira E C, Vilas-Boas L A, Alfieri A A
Laboratory of Animal Pathology, Universidade Estadual de Londrina, Paraná, Brazil.
Laboratory of Mycology, Universidade Estadual de Londrina, Paraná, Brazil.
J Comp Pathol. 2017 Jul;157(1):61-66. doi: 10.1016/j.jcpa.2017.06.001. Epub 2017 Jul 5.
The pathological and molecular findings associated with Talaromyces marneffei-induced pneumonia with concomitant infection by canine distemper virus (CDV) are described in a dog. The principal pathological alteration occurred in the lungs. Histopathology confirmed multifocal granulomatous pneumonia associated with numerous intralesional and intracellular septate fission cells consistent with T. marneffei. A molecular assay designed to amplify a partial fragment of the 18S rRNA gene of T. marneffei provided positive results from two fungal cultures derived from the lung. Sequencing and phylogenetic analyses confirmed the results of polymerase chain reaction (PCR). Furthermore, antigens of the CDV N protein were identified within the bronchial epithelium by immunohistochemistry and a PCR assay amplified the CDV N gene from hepatic and pulmonary fragments. Collectively, the pathological and molecular techniques confirmed a diagnosis of T. marneffei-induced pneumonia with concomitant infection by CDV. These findings represent the first description of pulmonary penicilliosis in the dog and extend the geographical niche of this emerging infectious pathogen. In this case, infection by CDV may have induced immunosuppression, which facilitated the development of pulmonary penicilliosis.
本文描述了一只犬感染马尔尼菲篮状菌（Talaromyces marneffei）并伴发犬瘟热病毒（CDV）感染所引起肺炎的病理和分子学发现。主要病理改变发生在肺部。组织病理学证实为多灶性肉芽肿性肺炎，伴有许多与马尔尼菲篮状菌一致的病灶内和细胞内分隔裂殖细胞。一项旨在扩增马尔尼菲篮状菌18S rRNA基因部分片段的分子检测，从两份源自肺部的真菌培养物中获得了阳性结果。测序和系统发育分析证实了聚合酶链反应（PCR）的结果。此外，通过免疫组织化学在支气管上皮内鉴定出了犬瘟热病毒N蛋白的抗原，并且PCR检测从肝脏和肺部片段中扩增出了犬瘟热病毒N基因。综合来看，病理和分子技术确诊了马尔尼菲篮状菌所致肺炎并伴有犬瘟热病毒感染。这些发现代表了犬肺青霉病的首次描述，并扩展了这种新出现的感染性病原体的地理分布范围。在该病例中，犬瘟热病毒感染可能诱导了免疫抑制，从而促进了肺青霉病的发展。
