Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, Campus Universitário, PO Box 10.011, Londrina, Paraná, 86057-970, Brazil.
Tissue Processing Unit, Multi-User Animal Health Laboratory, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
Braz J Microbiol. 2019 Jul;50(3):859-870. doi: 10.1007/s42770-019-00082-6. Epub 2019 Apr 17.
This report presents the pathologic findings associated with disseminated infection due to Cladosporium halotolerans in a dog that was simultaneously infected with canine adenovirus-1 (CAdV-1) and canine parvovirus-2 (CPV-2). A 12-year-old, mixed breed dog, with a clinical history of neurological manifestations was submitted for routine autopsy due to poor prognosis. The principal pathologic findings were mycotic necrotizing nephritis, hepatitis, and splenitis with embolic dissemination to the brain resulting in mycotic necrotizing meningoencephalitis, ventriculitis, choroid plexitis, and obstructive hydrocephalus associated with intralesional and intravascular septate pigmented fungi. PCR and sequencing of the ITS region of fungi revealed that the intralesional fungal organisms had 82% nucleotide identity with members of the Cladosporium sphaerospermum complex of organisms. However, a PCR assay and sequencing of the beta tubulin gene confirmed that the organism identified in this dog had 100% nucleotide sequence identity with C. halotolerans. Using immunohistochemistry, intralesional antigens of CAdV-1 were identified within the epithelial cells of the liver and lungs; there was positive immunolabeling for CPV-2 antigens in degenerated cardiomyocytes. These findings confirmed the active participation of C. halotolerans in the development of disseminated cladosporiosis in this dog and represent a rare occurrence of concomitant infection with CAdV-1 and CPV-2.
本报告介绍了一株耐盐枝孢菌（Cladosporium halotolerans）引起的犬感染的病理发现，该犬同时感染了犬腺病毒-1（CAdV-1）和犬细小病毒-2（CPV-2）。一只 12 岁的混种犬，有神经症状的临床病史，由于预后不良而进行常规尸检。主要的病理发现是真菌性坏死性肾炎、肝炎和脾炎，伴有栓塞性播散到大脑，导致真菌性坏死性脑膜脑炎、脑室炎、脉络丛炎和与腔内和血管内分隔色素真菌相关的阻塞性脑积水。对真菌的 ITS 区进行 PCR 和测序显示，腔内真菌与枝孢属 sphaerospermum 复合体的成员具有 82%的核苷酸同一性。然而，β-微管蛋白基因的 PCR 检测和测序证实，在这只狗中鉴定的生物体与耐盐枝孢菌具有 100%的核苷酸序列同一性。使用免疫组织化学，在肝脏和肺部的上皮细胞中鉴定到 CAdV-1 的腔内抗原；在退行性心肌细胞中检测到 CPV-2 抗原的阳性免疫标记。这些发现证实了耐盐枝孢菌在这只狗中发生播散性枝孢霉病的发展中的积极参与，并代表了同时感染 CAdV-1 和 CPV-2 的罕见情况。
