Jeong Je Hoon, Jin Eun-Sun, Kim Ji Yeon, Lee Bora, Min JoongKee, Jeon Sang Ryong, Lee Minsu, Choi Kyoung Hyo
Department of Neurosurgery, Soonchunhyang University Hospital, Bucheon, Korea; Laboratory of Stem Cell Therapy, College of Medicine, Asan Medical Center, University of Ulsan, Ulsan, Korea.
Department of Internal Medicine, College of Medicine, Kyung Hee University, Seoul, Korea; Laboratory of Stem Cell Therapy, College of Medicine, Asan Medical Center, University of Ulsan, Ulsan, Korea.
World Neurosurg. 2017 Oct;106:964-972. doi: 10.1016/j.wneu.2017.07.083. Epub 2017 Jul 20.
To evaluate the efficacy of biocomposite screws used in ovariectomy (OVX)-induced osteoporotic rats.
Twenty-four female Wistar rats (250-300 g, 12 weeks old) were divided into 4 groups: sham group (control), OVX-induced osteoporosis group (OVX), OVX and biodegradable poly(lactic-co-glycolic acid) (PLGA) without tricalcium phosphate (β-TCP) screw insertion group (OVX/BSR), and OVX and biocomposite (PLGA with β-TCP) screw insertion group (OVX/CSR). Three groups underwent bilateral OVX, and of these, 2 groups had 2 different types of screw inserted at the proximal tibia. At 25 weeks after OVX, the bilateral tibias were extracted. The extracted tibiae were scanned by ex vivo micro-computed tomography (CT) and stained with hematoxylin and eosin (H&E) and with Masson's trichrome stain for pathological assessment.
Compared with the ovariectomized groups, the control group had the highest values for bone mineral density (BMD), bone volume (BV)/total volume (TV), and trabecular number (Tb.N) and the lowest values for trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp). In the pairwise comparison among ovariectomized groups, the OVX/CSR group had significantly higher BMD, BV/TV, and Tb.N values than the other 2 groups (OVX and OVX/BSR) and significantly lower Tb.Sp. Micro-CT scans showed clear evidence of new trabecular formation near the screw insertion site in the OVX/CSR group only. Analyses of H&E- and Masson's trichrome-stained sections showed more and thicker trabecular bone around the implant in the OVX/CSR group compared with the OVX and OVX/BSR groups.
Biocomposite screws can improve local bone quality and facilitate osteoconductivity in an osteoporotic rat model.
评估生物复合螺钉用于卵巢切除（OVX）诱导的骨质疏松大鼠的疗效。
将24只雌性Wistar大鼠（250 - 300克，12周龄）分为4组：假手术组（对照组）、OVX诱导的骨质疏松组（OVX）、OVX且未插入磷酸三钙（β-TCP）螺钉的可生物降解聚乳酸-乙醇酸共聚物（PLGA）组（OVX/BSR）以及OVX且插入生物复合（PLGA与β-TCP）螺钉组（OVX/CSR）。三组接受双侧卵巢切除术，其中两组在胫骨近端插入两种不同类型的螺钉。OVX术后25周，取出双侧胫骨。取出的胫骨通过体外微型计算机断层扫描（CT）进行扫描，并用苏木精和伊红（H&E）以及马松三色染色进行病理评估。
与卵巢切除组相比，对照组的骨密度（BMD）、骨体积（BV）/总体积（TV）和骨小梁数量（Tb.N）值最高，骨小梁厚度（Tb.Th）和骨小梁间距（Tb.Sp）值最低。在卵巢切除组之间的两两比较中，OVX/CSR组的BMD、BV/TV和Tb.N值显著高于其他两组（OVX和OVX/BSR），Tb.Sp显著更低。微型CT扫描仅在OVX/CSR组的螺钉插入部位附近显示出新骨小梁形成的明确证据。对H&E和马松三色染色切片的分析显示，与OVX和OVX/BSR组相比，OVX/CSR组植入物周围的骨小梁更多且更厚。
生物复合螺钉可改善骨质疏松大鼠模型的局部骨质量并促进骨传导性。
