Armas Isabel, Pogrebnyak Natalia, Raskin Ilya
Department of Plant Biology, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901 USA.
Plant Methods. 2017 Jul 21;13:58. doi: 10.1186/s13007-017-0208-0. eCollection 2017.
Successful biotechnological improvement of crop plants requires a reliable and efficient in vitro regeneration system. Lettuce ( L.), one the most important vegetable crops worldwide, is strongly genotype-dependent in terms of regeneration capacity, limiting the potential for biotechnological improvement of cultivars which show recalcitrance under currently available protocols. The effect of different nutrient sources, plant hormone combinations and activated charcoal supplementation on shoot induction efficiency was evaluated on the cultivar 'RSL NFR', which had previously shown poor regeneration efficiency.
Multiple shoot organogenesis from cotyledon explants was recorded at the highest frequency and speed on Murashige and Skoog regeneration medium supplemented with 200 mg/l of activated charcoal, 3% sucrose, 10 mg/l benzylaminopurine and 0.5 mg/l naphthaleneacetic acid, which induced shoots through direct regeneration in 90.8 ± 7.9% of explants. High shoot induction efficiency was also observed, albeit not quantified, when using this medium on some other cultivars.
This activated charcoal-containing regeneration medium might offer a rapid and efficient option for direct shoot induction in some lettuce genotypes that do not respond well to common lettuce regeneration protocols. This is also the first report of the effect of activated charcoal in lettuce tissue culture.
作物的成功生物技术改良需要可靠且高效的离体再生系统。生菜是全球最重要的蔬菜作物之一，其再生能力在很大程度上依赖基因型，这限制了那些在现有方案下表现出顽拗性的品种进行生物技术改良的潜力。在先前再生效率较差的‘RSL NFR’品种上，评估了不同营养源、植物激素组合和添加活性炭对芽诱导效率的影响。
在添加了200毫克/升活性炭、3%蔗糖、10毫克/升苄氨基嘌呤和0.5毫克/升萘乙酸的Murashige和Skoog再生培养基上，子叶外植体的多芽器官发生频率和速度最高，该培养基通过直接再生在90.8±7.9%的外植体中诱导出芽。在其他一些品种上使用该培养基时，也观察到了较高的芽诱导效率，尽管未进行量化。
这种含活性炭的再生培养基可能为一些对常见生菜再生方案反应不佳的生菜基因型提供一种快速有效的直接芽诱导方法。这也是活性炭在生菜组织培养中作用的首次报道。
