Protasova Irina N, Wan Tsai-Wen, Bakhareva Natalya V, Hung Wei-Chun, Higuchi Wataru, Iwao Yasuhisa, Yelistratova Tatyana A, Ilyenkova Natalya A, Sokolovskaya Yelena S, Martynova Galina P, Reva Ivan V, Reva Galina V, Sidorenko Sergey V, Teng Lee-Jene, Peryanova Olga V, Salmina Alla B, Yamamoto Tatsuo
Russia-Japan Center of Microbiology, Metagenomics and Infectious Diseases, Krasnoyarsk, Russia.
Department of Microbiology Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
Microbiol Immunol. 2017 Sep;61(9):359-370. doi: 10.1111/1348-0421.12503.
Streptococcus pneumoniae, a common human pathogen, colonizes the nasopharynx and causes diseases including acute otitis media (AOM). Herein, pneumococcal serotype distributions in children before and after PCV7 vaccination and in patients with pneumococcal disease in Siberian Russia (Krasnoyarsk) are reported. Analyses included antimicrobial susceptibility testing, sequence typing (ST), pulsed field gel electrophoresis, virulence-related surface protein gene (VSG) typing with novel primers and structural analysis by scanning electron microscopy. In healthy children (HC) prior to administration of PCV7, drug-susceptible serotype23F/ST1500 was a major pneumococcal genotype. In the PCV7 trial, multidrug-resistant serotype19A/ST320 emerged in vaccinees after PCV7, exhibiting a PCV7-induced serotype replacement. Multidrug-resistant serotype19A/ST320 was evident in patients with AOM. Community-acquired pneumonia (CAP) isolates showed genetic similarities to the AOM (ST320) genotype, constituting a common non-invasive AOM-CAP group. In contrast, meningitis isolates were more divergent. Overall, 25 ST types were identified; five (20%) of which were Krasnoyarsk-native. Regarding VSGs, PI-1 (rlrA/rrgB), PI-2 (pitA/B), psrP and cbpA were present at 54.3%, 38.6%, 48.6%, and 95.7%, respectively, with two major VSG content types, PI-1 /PI-2 /psrP /cbpA and PI-1 /PI-2 /psrP /cbpA , being found for HC and non-invasive diseases, respectively. A major clone of serotype19A/ST320 (PI-1 /PI-2 ) produced the longest pneumococcal wire (pilus) structures in colonies. ST1016 (PI-1 /PI-2 ) in HC had HEp-2 cell-adherent pili. These results suggest that serotype19A/ST320 and related genotypes, with the VSG content type PI-1 /PI-2 /psrP /cbpA , emerged in vaccinees after PCV7 in Siberia, accompanying diseases in non-vaccinated children, and that some genotypes (serotypes19A/ST320 and 18/ST1016) produced novel pneumococcal structures, predicting their roles in colony formation and adherence.
肺炎链球菌是一种常见的人类病原体，可定植于鼻咽部并引发包括急性中耳炎（AOM）在内的多种疾病。本文报告了俄罗斯西伯利亚地区（克拉斯诺亚尔斯克）儿童接种PCV7疫苗前后以及肺炎球菌疾病患者的肺炎球菌血清型分布情况。分析内容包括抗菌药敏试验、序列分型（ST）、脉冲场凝胶电泳、使用新型引物进行毒力相关表面蛋白基因（VSG）分型以及通过扫描电子显微镜进行结构分析。在接种PCV7疫苗之前的健康儿童（HC）中，药敏血清型23F/ST1500是主要的肺炎球菌基因型。在PCV7试验中，接种PCV7疫苗后的受种者中出现了多重耐药血清型19A/ST320，呈现出PCV7诱导的血清型替换现象。多重耐药血清型19A/ST320在AOM患者中出现。社区获得性肺炎（CAP）分离株与AOM（ST320）基因型具有遗传相似性，构成了一个常见的非侵袭性AOM-CAP组。相比之下，脑膜炎分离株差异更大。总体而言，共鉴定出25种ST型；其中五种（20%）是克拉斯诺亚尔斯克本地特有的。关于VSG，PI-1（rlrA/rrgB）、PI-2（pitA/B）、psrP和cbpA的出现率分别为54.3%、38.6%、48.6%和95.7%，在HC和非侵袭性疾病中分别发现了两种主要的VSG含量类型，即PI-1 /PI-2 /psrP /cbpA和PI-1 /PI-2 /psrP /cbpA。血清型19A/ST320的一个主要克隆（PI-1 /PI-2）在菌落中产生了最长的肺炎球菌丝（菌毛）结构。HC中的ST1016（PI-1 /PI-2）具有黏附于HEp-2细胞的菌毛。这些结果表明，在西伯利亚地区，接种PCV7疫苗后的受种者中出现了血清型19A/ST320及相关基因型，其VSG含量类型为PI-1 /PI-2 /psrP /cbpA，并伴随着未接种疫苗儿童的疾病发生，而且一些基因型（血清型19A/ST320和18/ST1016）产生了新型肺炎球菌结构，预示着它们在菌落形成和黏附中的作用。
