从植物提取物中分离氨基酸和酰胺氮用于¹⁵N分析。

Separation of amino acid and amide nitrogen from plant extracts for 15N analysis.

作者信息

Ta T C, Joy K

出版信息

Anal Biochem. 1986 May 1;154(2):564-9. doi: 10.1016/0003-2697(86)90030-8.

Abstract

A rapid method was developed to obtain nitrogen for 15N analysis of individual amino acids and amides from plant tissue extracts. Amino or amide nitrogen was recovered as ammonia, suitable for preparation of samples for 15N emission spectrometry, using a combination of ion-exchange chromatography and distillation.

摘要

开发了一种快速方法，用于从植物组织提取物中获取氮，以对单个氨基酸和酰胺进行¹⁵N分析。利用离子交换色谱法和蒸馏法相结合，将氨基或酰胺态氮以氨的形式回收，适用于制备¹⁵N发射光谱分析的样品。