植物组织提取物中游离铵、天冬酰胺及谷氨酰胺酰胺氮的测定。
Determination of free ammonium and asparagine and glutamine amide-nitrogen in extracts of plant tissue.
作者信息
Henderlong P R, Schmidt R R
出版信息
Plant Physiol. 1966 Sep;41(7):1102-5. doi: 10.1104/pp.41.7.1102.
A relatively simple and rapid procedure for the measurement of free ammonium and the amides in plant extracts is described. The method was developed by combining a cation-exchange method for blood ammonia with a differential acid-hydrolysis procedure for asparagine and glutamine amide-nitrogen. The recovery of standard samples (100-400 mug of ammonium- or amide-nitrogen) of free ammonium, asparagine, and glutamine after being run through the extraction, column, and analytical procedures ranged between 99 and 102%. The harvest, extraction, and analytical procedures were tested on shoots from 4 to 6-day-old germinating barley seeds. The high levels of the amides and the low level of free ammonium present in the tissue extracts indicated that the extraction and analytical procedures resulted in little if any hydrolysis of the amides.
本文描述了一种相对简单快速的方法，用于测定植物提取物中的游离铵和酰胺。该方法是通过将血液氨的阳离子交换法与天冬酰胺和谷氨酰胺酰胺氮的差示酸水解法相结合而开发的。游离铵、天冬酰胺和谷氨酰胺的标准样品（100 - 400微克铵或酰胺氮）经过提取、柱分离和分析程序后的回收率在99%至102%之间。对4至6日龄发芽大麦种子的芽进行了收获、提取和分析程序测试。组织提取物中酰胺含量高而游离铵含量低，这表明提取和分析程序导致酰胺几乎没有水解。
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