Yang Kunlong, Qin Qiuping, Liu Yinghang, Zhang Limei, Liang Linlin, Lan Huahui, Chen Chihao, You Yunchao, Zhang Feng, Wang Shihua
Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University Fuzhou, China.
Front Cell Infect Microbiol. 2016 Dec 21;6:190. doi: 10.3389/fcimb.2016.00190. eCollection 2016.
is one of the most important opportunistic pathogens of crops and animals. The carcinogenic mycotoxin, aflatoxins produced by this pathogen cause a health problem to human and animals. Since cyclic AMP signaling controls a range of physiological processes, like fungal development and infection when responding to extracellular stimuli in fungal pathogens, in this study, we investigated the function of adenylate cyclase, a core component of cAMP signaling, in aflatoxins biosynthesis and virulence on plant seeds in . . A gene replacement strategy was used to generate the deletion mutant of that encodes the adenylate cyclase. Severe defects in fungal growth, sporulation and sclerotia formation were observed in the deletion mutant. The defect in radical growth could be partially rescued by exogenous cAMP analog. The mutant was also significantly reduced in aflatoxins production and virulence. Similar to the former studies in other fungi, The mutant showed enhancing tolerance to oxidative stress, but more sensitive to heat stress. Overall, the pleiotropic defects of the deletion mutant indicates that the cAMP-PKA pathway is involved in fungal development, aflatoxins biosynthesis and plant seed invasion in .
是农作物和动物最重要的机会性致病病原体之一。这种病原体产生的致癌霉菌毒素黄曲霉毒素会给人类和动物带来健康问题。由于环磷酸腺苷（cAMP）信号传导控制一系列生理过程，如真菌病原体在响应细胞外刺激时的真菌发育和感染，在本研究中，我们研究了cAMP信号传导的核心成分腺苷酸环化酶在黄曲霉毒素生物合成以及对植物种子的毒力方面的功能。采用基因替换策略构建了编码腺苷酸环化酶的缺失突变体。在该缺失突变体中观察到真菌生长、孢子形成和菌核形成存在严重缺陷。外源cAMP类似物可部分挽救其根部生长缺陷。该突变体在黄曲霉毒素产生和毒力方面也显著降低。与之前在其他真菌中的研究类似，该突变体表现出对氧化应激的耐受性增强，但对热应激更敏感。总体而言，该缺失突变体的多效性缺陷表明cAMP - PKA途径参与了该真菌的发育、黄曲霉毒素生物合成以及对植物种子的侵染。
